不同生长年限及不同部位川明参红外光谱研究

The Infrared Spectroscopic Study of Radix Chuanmingshinis Violacei with different growing periods and Different Parts

目的对不同生长年限及不同部位川明参Chuanmingshen violaceum Sheh et Shan进行红外光谱及二阶导数图谱研究。方法采用溴化钾压片法和傅里叶变换红外光谱法对不同生长年限川明参及川明参根茎和茎叶进行红外光谱及二阶导数图谱进行研究。结果随着生长年限的增长,川明参次生代谢所累积的物质逐渐增多,在3366cm^(-1)、2927cm^(-1)、1634cm^(-1)、1416cm^(-1)、1516cm^(-1)、1080cm^(-1)、1020cm^(-1)和928cm^(-1)等位置出现特征吸收峰,主要化学成为多糖、香豆素、皂苷和甾体等。根茎和茎叶化学成分差异明显。结论采用红外光谱法对不同生长年限及不同部位川明参进行研究,图谱特征明显,方法简便,适合川明参的鉴别与质量评价。

Objective Do infrared spectroscopic study of different growth years and different parts of the traditional Chinese medicine Chuanmingshen violaceum Sheh et Shan. Methods Using potassium bromide squash method and Fourier transform infrared spectroscopy on the different growth years and different parts of Radix Chuanmingshinis Violacei for infrared spectrum and second derivative spectrum. Results With the increasing of growth time, the Radix Chuanmingshinis Violacei accumulated in the secondary metabolism substances gradually increased, the characteristic absorption peak appear in the position such as 3366cm^-1, 2927cm^-1, 1634cm^-1, 1416cm^-1, 1516cm^-1,1080cm^-1,1020cm^-1,928cm^-1, the main chemical as polysaccharide, coumarin, saponins and steroids. Root tuber and cauline leaf chemical composition difference is obvious. Conclusion Using infrared spec- troscopy in different growing periods and different parts of Radix Chuanmingshinis Violacei were studied, of obvious characteristics, identification and quality evaluation method is simple and suitable for the Radix Chuanmingshinis Violacei.