杜仲种质资源雄花主要数量性状变异及概率分级

Variation and probability grading of main quantitative traits of male flowers for Eucommia ulmoides germplasm

为更好地对杜仲种质资源雄花进行评价和利用,对193份不同种质杜仲雄花14个主要数量性状进行变异分析和概率分级。结果表明：杜仲雄花数量性状存在丰富的变异,14个性状变异系数均在12%以上,京尼平苷含量（112.00%）变异系数最大,其次为紫云英苷含量（89.56%）、京尼平苷酸含量（88.60%）,氨基酸含量（12.52%）变异系数最小。经K-S检验,花高、雄蕊长度、雄蕊数、桃叶珊瑚苷含量、绿原酸含量、异槲皮苷含量、总黄酮含量、氨基酸含量等8个数量性状Sig值大于0.05,均符合正态分布;花径、鲜质量、干质量均呈偏态分布,去除拖尾部分,也近似看作正态分布;京尼平苷酸含量、京尼平苷含量、紫云英苷含量均不符合正态分布。对符合正态分布的数量性状统一用（X-1.281 8S）、（X-0.524 6S）、（X＋0.524 6S）、（X＋1.281 8S）4个点分为5级,对不符合正态分布的性状按实际分布情况分级,使1～5级出现的概率分别为10%、20%、40%、20%和10%。

To make better evaluation and utilization,14 main quantitative traits of 193 male flowers of Eucommia ulmoides germaplasm were studied through variation analysis and probability grading. The results showed that a large variation was observed in the quantitative traits of the flowers,coefficients of variation of the 14 quantitative traits were all above 12%,in which the highest occurred in geniposide content（ 112. 00%）, followed by astragaline content（ 89. 56%） and geniposidic acid content（ 88. 60%）,and the smallest occurred in amino acids content（ 12. 52%）. According to the K-S test,flower height,stamen length,stamen number,aucubin content,chlorogenic acid content,isoquercitrin content,total flavonoids content and amino acid content all conformed to the normal distribution; flower diameter,fresh weight and dry weight that followed skewed distribution could be regarded and treated as those conforming to normal distribution; geniposidic acid content,geniposide content and astragaline content did not conform to normal distribution. Eleven quantitative traits that conformed to the normal distribution could be divided into 5 grades by 4 dividing points： X- 1. 281 8S,X- 0. 524 6S,X ＋0. 524 6S and X ＋ 1. 281 8S,while other 3 traits could be divided into 5 grades according to the actual distribution. The occurrence probability of grade 1- 5 was 10%,20%,40%,20% and 10%,respectively. An index system of probability grading for 14 quantitative traits was built,which could provide important guidelines for E. ulmoides breeding program and quality control of male flower related products,and meanwhile,it could provide a reference for specification and data standardization of E. ulmoides male flowers descriptor system.