山西省壳囊孢属真菌分类学

Taxonomy of Cytospora in Shanxi Province,northern China

本文通过形态学及系统发育学方法对采自中国山西省不同地区的58份壳囊孢属真菌标本进行分类学研究。从形态学上观察子实体、孢子及纯培养物形态,可将其分为5个形态学类群;系统发育学分析分别基于单基因(ITS)和3个基因片段数据集(ITS、LSU和TEF),通过最大简约法(MP),最大似然法(ML)和贝叶斯分析(BI)分别构建系统发育树,结果显示来自山西的壳囊孢属真菌为5个独立分支,进一步支持了形态学分析结果所得出的结论。本文结合传统形态学及现代分子系统学方法对山西省不同寄主壳囊孢属真菌多样性进行调查和分类研究,经分析该5个类群分别是榆树壳囊孢、金黄壳囊孢、核果壳囊孢、苹果壳囊孢、槐壳囊孢,分布于山西省5个市的11种寄主植物上,其中广玉兰、山楂、白蜡、丝绵木、红瑞木为该类真菌新记录寄主,为该省病害防治及后续病原病理学研究奠定基础。

Morphological and phylogenetic approaches were employed to examine the diversity of 58 Cytospora specimens collected from different regions of Shanxi Province in northern China. The results showed presence of five morphological groups according to characteristics of fruiting body,spore shape and pure cultures. Three different algorithms were used to construct phylogenetic trees based on sequences of a single gene（ ITS） and three genes（ ITS,LSU and TEF）,which showed that Cytospora from Shanxi could be clustered into five separate clades,further supporting the morphological results. The five groups were identified as C. carbonacea,C. chrysosperma,C. leucostoma,C. mali and C. sophorae,which were isolated from 11 different hosts of five cities of Shanxi Province. The hosts Crataegus pinnatifida,Euonymus bungeanus, Fraxinus chinensis and Magnolia grandiflora were found for the first time associated with Cytospora canker, which is critical to the disease prevention and treatment, and subsequent studies of pathogen pathology.