β-榄香烯对人胃癌细胞SGC7901的抑制作用及部分机制

Study of β-elemene on tumor inhibition and partial mechanism of human gastric cancer SGC7901 cell

目的研究β-榄香烯对胃癌细胞SGC7901的增殖、凋亡、迁移及赖氨酰氧化酶（LOX）的影响。方法体外培养人胃癌SGC7901细胞,MTS法及流式细胞术检测不同浓度β-榄香烯对SGC7901细胞增殖与凋亡的影响,划痕实验、Transwell小室迁移实验检测β-榄香烯对细胞迁移的影响,酶标仪法检测β-榄香烯对LOX酶活性的影响,Western blot法检测LOX蛋白表达的变化。结果β-榄香烯（50~800μmol/L）对SGC7901细胞的抑制作用呈时间依赖和浓度依赖性。β-榄香烯（100、200、400、800μmol/L）处理SGC7901细胞24 h后,凋亡率分别为8.1%、8.2%、18.7%、41.9%,与对照组（4.9%）比较,凋亡率明显升高（P〈0.05）。划痕实验、Transwell实验结果显示β-榄香烯对SGC7901细胞的迁移有明显抑制作用（P〈0.05）。β-榄香烯浓度依赖性地降低SGC7901细胞LOX酶活性（P〈0.05）。Western blot结果显示β-榄香烯可下调SGC7901细胞LOX的蛋白表达。结论β-榄香烯对胃癌细胞SGC7901有明显抑制增殖、迁移及促进凋亡作用,抑制LOX酶活性和蛋白表达可能是其发挥作用的机制之一。

Objective To investigate the inhibitory effect of β-elemene on the proliferation,apoptosis,migration and LOX activity of the gastric cancer SGC7901 cell line. Methods MTS was performed to reveal the inhibitory effect on cell proliferation with different concentrations β-elemene on SGC7901 cell,flow cytometry was used to determine the apoptosis of the gastric cancer SGC7901 cell line after the treatment of β-elemene. Effects of β-elemene on the migration of SGC7901 cells were detected by wound healing assay and transwell assay respectively. Changes of LOX activity were determined by colorimetric method,and changes of LOX expression were determined by Western blot.Results β-elemene（ 100,200,400,800 μmol/L） significantly inhibited the proliferation on SGC7901 cells in a time and dose-dependent manner. After β-elemene treatment for 24 h in SGC7901 cells,the apoptotic ratios were8. 1%,8. 2%,18. 7% and 41. 9% with concentrations of β-elemene at 100,200,400,800 μmol/L. Compared with the control（ 4. 9%）,apoptosis rate increased obviously. Effects of migration on SGC7901 cells were significantly inhibited by Wound healing assay and Transwell assay. LOX activity was decreased in a dose-dependent manner.Western blot showed that β-elemene SGC7901 cells could down-regulate expression of LOX. Conclusion The proliferation,apoptosis and migration of the gastric cancer SGC7901 cell line can be inhibited after the treatment of β-elemene and the molecular mechanism for those effects may be related to its down-regulated LOX activity and the express of LOX.