相对时间法计算凝胶渗透色谱测试大分子分子量和分子量分布

The New Method of Calculating the Molecular Weight and Distribution of Macromolecules in GPC Test by Using Relative Time Method

在凝胶渗透色谱(GPC)对大分子的分子量和分子量分布的测试中,本研究利用小分子乙二醇在系统中出峰时间恒定的现象,采用乙二醇的出峰时间作为标识物,将GPC的色谱图转化为相对时间,再以相对时间建立校正曲线,作为计算分子量和分子量分布的依据。此方法解决了在测试色谱图的记录、校准曲线的建立和分子量的计算中以实际时间来表示和计算分子量时,由于GPC泵的重复性不稳定、色谱柱柱效的下降、仪器更换过不同的色谱柱等因素造成的使校正曲线出现偏差、分子量计算结果出现误差等问题。

In the test of the macromolecular weight and molecular weight distribution by Gel permeation chromatography （GPC） ,according to the phenomenon that the micromolecule ethylene glycol＇ s peak time is constant in the system, this research used ethylene glycol＇s peak time as a marker, The chromatographs under the relative time are used to establish a calibration curve which istransformed from those under the absolute time. This project solves the problems caused by the repeatability of GPC instrument＇s instability, the declination of chromatographic column efficiency and the replacement of chromatographic column in the records of chromatogram, the establishment of calibration curve and the calculation of molecular weight under the real time.