没食子提取物对IgA肾病大鼠转化生长因子-β_1的影响

Effect of Turkish galls extract on the expression of transforming growth factor-β_1 in IgA nephropathy rats

目的研究不同剂量没食子提取物(TGE)对Ig A肾病(IgAN)大鼠的血清、尿液及肾组织的转化生长因子-β_1(TGF-β_1)水平的影响。方法 50只SD大鼠随机分为正常组、模型组、高中低3个剂量(TGE:300,150,75mg·kg^(-1))实验组,每组各10只。给药第12,16周末,以BCA蛋白定量法测定24 h尿蛋白。第16周末,用全自动生化分析仪测定血肌酸酐(Scr)、尿素氮(BUN)含量;以酶联免疫吸附法测定血清及尿液中的TGF-β_1水平;用免疫组织化学法测定肾组织TGF-β_1水平;以免疫荧光法测定肾小球Ig A沉积强度;并用电镜观察各组大鼠肾组织改变。结果给药第16周末,与模型组大鼠的尿液TGF-β_1水平(243.25±50.46)ng·mL^(-1)相比,高中低3个剂量实验组大鼠尿液TGF-β_1水平分别为(137.82±14.53),(150.25±21.32),(185.79±17.12)ng·mL^(-1),显著降低,差异有统计学意义(均P<0.05);高、中2个剂量实验组均比低剂量实验组水平低,差异有统计学意义(P<0.05)。第16周末,与正常组血清TGF-β_1水平(21.70±3.62)ng·mL^(-1)相比,模型组大鼠血清的TGF-β_1水平为(90.88±19.63)ng·mL^(-1),显著升高,差异有统计学意义(P<0.05);而高中低3个剂量实验组大鼠血清TGF-β_1水平分别为(38.01±7.15),(43.81±7.27),(57.90±8.70)ng·mL^(-1),较模型组降低,差异有统计学意义(P<0.05)。与模型组相比,仅有高剂量实验组TGF-β_1表达减弱(Z=-3.063,P<0.01)。与模型组比较,高、中2个剂量实验组得免疫荧光评分较低,Ig A沉积较弱(高中低3个剂量实验组:Z=-3.243,P<0.001;Z=-3.072,P<0.01;Z=-1.980,P>0.05),高、中2个剂量差异有统计学意义(P<0.01)。结论 TGE能降低Ig AN大鼠血尿及肾组织TGF-β_1水平,减弱肾组织Ig A沉积强度,起到一定肾保护作用。

Objective To observe the effect of Turkish galls extract （TGE） on expression of transforming growth factor - β1 （TGF - β1 ） in IgA nephropathy （IgAN） rats. Methods A total of 50 SD rats were ran- domly divided into normall group, model group, high dose experimental group , medium dose experimental group and low dose experimental group, 10 rats in each group. The 24 hour urine protein were determined by BCA protein quantitative at the end of week - 12 and week - 16. At the end of week - 16, serum ereatinine （Scr） and blood urea nitrogen （BUN） were measured by automatic biochemistry analyzer. The expre- ssion of TGF -β1 in serum and urine were measured by enzyme linked immunosorbent assay. The expression of TGF - β1, in renal tissue were determined by immunohistochemistry. The depo- sition intensity of glomerular IgA were determinated by immunofluorescence. The renal morphology and uhrastructure were examined by electron microscopy. Results At the end of week - 16 after administration, the serum TGF - β1 in model group was （90. 88± 19.63） ng · mL^-1 and urinary TGF- β1 was（243.25 -＋50. 46）ng · mL^-1, compared with model group, three dose groups （300, 150, 75 ） mg · kg^-1 of serum TGF- 131levels in three dose groups were （38.01 ±7.15）, （43.81±7.27）, （57.90 ±8.70） ng· mL^-1, urinary TGF - 131 were （137.82 ±14.53）, （150. 25 ±21.32）, （185.79 ± 17.12 ）ng · mL^-1, the difference had significance （all P 〈 0. 05 ）. Compared with model group, the expression of TGF- β1 in high doses experimental group （Z = -3.063 ,P 〈0. 01 ）. The deposition intensity of glomerular IgA in high and medium dose experimental groups （ Z = - 3. 243, P 〈 0. 001 ; Z = - 3. 072, P 〈 0. 01 ） were significantly weakened. Conclusion TGE can reduce the levels of TGF - β1 in serum, urine and renal tissue in IgAN rats;weaken the deposition intensity of glomerular IgA and play a kidney protection.