基因芯片技术在煤工尘肺合并肺结核中的应用价值

Diagnostic value of gene clip for coal workers' pneumoconiosis complicated with pulmonary tuberculosis

目的研究基因芯片技术在煤工尘肺合并结核诊断中的应用。方法所有病人均留取合格痰液,通过基因芯片技术做分枝杆菌菌种鉴定,如为结核分枝杆菌,则做耐药基因检测。结果结果显示,结核分枝杆菌阳性病人共15人,阳性率为60%,非结核分枝杆菌病人共10人,阳性率为40%,结核分枝杆菌中有1例对异烟肼耐药,耐药率为6.7%。在10例非结核分枝杆菌中,胞内分枝杆菌6例,占60%,结核分枝杆菌复合群1例,占10%,龟脓或脓肿分枝杆菌1例,占10%。结论胞内分枝杆菌为非结核分枝杆菌主要致病菌种;基因芯片技术具有简便、快速等特点,可准确区分结核分枝杆菌、非结核分枝杆菌,并可鉴定至种或群,可做耐药监测,可为临床诊断、治疗提供有力的支持。

Objective To explore the diagnostic value of gene clip for coal workers＇ pneumoconiosis complicated with pulmonary tuberculosis. Methods All the patients were used to detect Mycobacterium tuberculosis by gene clip. Mycobacterium strains isolated from all the patients were identified by drug-resistant genes. Results 15 strains of Mycobaterium isolated were M. tuberculosis, accounting for 60%. 10 strains were non-tuberculosis Mycoberium, accounting for 40%. In the 15 strains of M. tuberculosis, 1 strain was resistant to isoniazid （6. 7% ）. 6 strains were M. intracellulare of all the non-tuberculosis Mycobacterium （60%）. 1 strain was M. tuberculosis complex group, accounting for 10%. 1 strain was M. chelonae （ 10% ）. 2 strains were non-tuberculosis Mycobacterium, but they couldn＇ t be identified. Conclusion Of all the coal workers＇ pneumoconiosis complicated with non-tuberculosis Mycobacterium, the main strain is M. Intracellulare. The gene clip is simple, rapid, sensitive and specific for detecting tuberculosis. It could diagnose M. tuberculosis and non-tuberculosis Mycoberium and monitor drug resistance, which could be taken as a helpful method for diagnosis and treatment of pulmonary tuberculosis.