摘要
目的 从分子水平上分析对比天麻的3个不同变型,探索其种内变异,为资源保护和良种选育奠定基础。方法 采用SRAP分子标记分析天麻3个不同变型的遗传关系。结果 筛选出7对SRAP引物,用PCR扩增11批天麻样品,得到145条清晰的带,其中110条具有多态性,多态百分率为75.86%;通过Nei＆Li遗传相似系数（GS）计算,11批天麻的种质两两间的相似系数分布在0.36～0.93;UPGMA聚类分析将乌天麻聚为一类,红天麻与绿天麻聚为一类。结论 天麻的3个不同变型中,绿天麻和红天麻的亲缘关系较近,而乌天麻与前两者的遗传差异较大,亲缘关系较远。
OBJECTIVE To analyze the genetic relationship among three different types of Gastrodia elata. To select improved varie- ties and protect resources. METHODS SARP was used to analyze the genetic relationships. RESULTS Seven pairs of SARP mark- ers produced 145 loci, in which 110 were polymorphic loci. The total percentage of polymorphic loci was 75.86%. The Nei＇s genetic identity was 0. 36 - 0. 93. UPGMA clustering results showed that G. elata, f. elata and G. elata, f. viridis grouped together, while G. elata, f. glauca were clustered into a group. CONCLUSION The relationship between G. elata, f. elata and G. elata, f. viridis is closer, and they shows higher genetic uniformity with G. elata, f, glauca.
出处
《华西药学杂志》
CAS
CSCD
2016年第6期593-595,共3页
West China Journal of Pharmaceutical Sciences
基金
四川省科技富民强县专项计划(2013)
成都中医药大学自然科学基金(030021039)
关键词
天麻
变型
SRAP
遗传关系
乌天麻
红天麻
绿天麻
Gastrodia elata
Different variants
SRAP
Genetic relationship
G. elata, f. glauca
G. elata, f. viridis
G. elata, f. elata
