摘要
目的采用HPLC法测定紫蒲痛风合剂中绿原酸的含量。方法采用Waters Symmetry Shield RP18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.4%磷酸溶液(10∶90),流速1.0 m L·min^(-1),检测波长327 nm。结果 67.2~806.4 ng绿原酸与峰面积呈良好的线性关系(r=0.9999),平均加样回收率为115.54%,RSD=0.92%(n=6)。结论所用方法准确、简便、可靠,可用于该制剂的质量控制。
OBJECTIVE To determine the chlorogenic acid in Zipu Tongfeng mixture (ZTM) by HPLC. METHODS A Waters Symmetry Shield RP18 column( 250 mm ×4.6 mm, 5 μm)was selected with a mobile phase of acetonitrile -0.4% phosphoric acid solution (10 : 90). The detection wavelength was 327 nm and the flow rate was 1.0 mL·min^-1. RESULTS The Chlorogenic acid had fine linear responses ( r = 0.9999 ) in the concentration range of 67.2 - 806.4 ng. The recovery was 115.54% and the RSD was 0.92% ( n = 6). CONCLUSION The method is accurate, simple and reliable. And it can be used to control the quality of ZTM.
出处
《华西药学杂志》
CAS
CSCD
2016年第6期638-639,共2页
West China Journal of Pharmaceutical Sciences
基金
四川省科技支撑计划(2014SZ0141)
关键词
紫蒲痛风合剂
含量测定
质量控制
绿原酸
忍冬藤
Zipu Tongfeng mixture
Quantity determination
Quality control
Chlorogenic acid
Caulis lonierae