SF-1诱导小鼠子宫内膜基质细胞增殖及其机制的研究

SF-1 Induces the Proliferation of Mouse Endometrial Stromal Cells In Vitro and Its Mechanism

该研究分离并鉴定了原代小鼠子宫内膜基质细胞(mouse endometrial stromal cell,m ESC)。过表达类固醇生成因子-1(steroidogenic factor-1,SF-1)可显著诱导该细胞的增殖能力、促进增殖相关基因PCNA(proliferating cell nuclear antigen)及PH3(phospho-histone 3)的蛋白质水平表达增高以及激活PI3K-AKT-m TOR信号通路;PI3K信号通路抑制剂LY294002和m TOR信号通路抑制剂Rapamycin等可抑制mESC中SF-1过表达所引起p AKT S473、pm TOR S2448、pp70S6K T389、p S6 S235/236和p4E-BP1 T37/46的蛋白质水平的增高。该研究初步揭示了SF-1表达与mESC增殖的相关性。

Mouse endometrial stromal cell （mESC） was isolated and identified in this study. Forced expression of steroidogenic factor-1 （SF-1） in mESC induced proliferation increase and upregulation of protein expression in proliferation marker genes PH3 （phospho-histone 3） and PCNA （proliferating cell nuclear antigen） and acitivation of PI3K-AKT-mTOR signaling pathway. The PI3K signaling pathway inhibitor LY294002 and roTOR signal pathway inhibitor Rapamycin decreased the expression of pAKT S473, pmTOR $2448, pp70S6K T389, pS6 S235/236 and p4E-BP1 T37/46, which were induced by forced expression of SF-1 in primary cultured mESC. Our study provided preliminary evidences that SF-1 expression might associated with mESC cell proliferation.