小鼠失血性休克模型的建立与比较

Establishment and comparison of mouse models for hemorrhagic shock

目的建立3种小鼠失血性休克模型,比较血压、血气、血细胞计数等指标,为失血性休克相关实验动物模型的选择提供依据。方法将32只雄性BALA/C小鼠随机均分为失血30%控量组(V1组)、失血40%控量组(V2组)、35-40 mm Hg控压组(P组)和对照组(Control组),通过股动脉插管放血制作失血性休克模型,连续监测血压,90 min时取动脉血进行血气分析和血细胞计数。结果 Control、P组平均动脉压保持稳定,分别为(90.4±1.2)mmHg、(37.8±1.0)mm Hg,血压波动性分别为(3.05±0.16)%、(2.64±0.23)%;V1、V2组平均动脉压均在放血完毕后5 min左右进入平台期,分别为(64.0±2.9)mm Hg、(47.2±3.4)mm Hg,血压波动性分别为(13.66±2.29)%、(16.57±3.01)%;血气检测结果中Control、V1、V2、P组相比较,Hb(g/d L)为14.9±0.7、11.9±0.4、11.2±0.3和10.4±0.6(P<0.01),Hct(%)为45.5±2.1、36.5±1.2、34.4±1.0和32.0±1.6(P<0.01),Lac(mmol/L)为3.6±0.8、2.4±0.4、3.6±0.3和5.0±1.1(P<0.01),K+(mmol/L)为4.2±0.3、4.0±0.3、5.0±0.2和5.6±0.8(P<0.01);血细胞计数结果中Control、V1、V2、P组相比较,RBC(10×12/L)为10.58±0.55、8.84±0.42、8.39±0.46和7.71±0.54(P<0.01),WBC(10×9/L)为8.24±2.54、4.32±1.06、3.57±0.75和4.12±0.80(P<0.01),Plt(10×9/L)为724±80、661±103、577±66和607±94(P>0.05)。结论失血30%、40%控量组血流动力学变化过程接近临床,适于失血性休克后代谢情况、病理生理、存活情况及治疗策略的研究。35-40 mm Hg控压组休克程度重、血压波动性较小,适于失血性休克后代谢性酸碱平衡紊乱、药效学、器官损伤及其分子机制的研究。

Objective To establish three mouse models of hemorrhagic shock, to compare blood pressure, blood gas and blood cell count results, and to provide evidence for the selection of related experimental animal models. Methods All 32 male BALA/C mice were randomly divided into 30% volume-fixed group （V1） , 40% volume-fixed group （V2） , 35 -40 mmHg pressure-fixed group （P） and a control group. The hemorrhagic shock models were created through femoral artery in- tubation and hemorrhage. The blood pressure was continuously monitored for 90 minutes. Arterial blood samples were taken for blood gas analysis and blood cell count. Results Of the control and P groups, the mean arterial pressure remained stable and were （90. 4 ± 1.2 ） mmHg and （ 37. 8 ± 1.0） mmHg, respectively. The blood pressure variability were （ 3.05 ± 0. 16） % and （2. 64 ± 0. 23） %. Of the V1 and V2 groups, the mean arterial pressure entered plateau period in 5 minutes after bleeding and were recorded as （64. 0 ± 2. 9） mmHg and （47.2 ± 3.4） mmHg, respectively. The blood pressure varia- bility were （ 13.66 ± 2.29 ） % and （ I6. 57 ±3.01 ） %. Comparing the results of blood gas analysis between control, V 1, V2 and P groups, the Hb （g/dL） contents were 14.9 ± 0.7, 11.9 ~ O. 4, 11.2 ~ 0. 3 and 10.4 ~ O. 6 （ P 〈 0.01 ）, the Hct （%） were 45.5 ±2. 1, 36.5 ±1.2, 34.4±1.0 and 32.0±1.6 （P〈0.01）, the Lac （mmol/L） were 3.6 ±0.8, 2.4 ~ 0.4, 3.6±0.3 andS. 0±l.l （P〈0.01）, the （retool/L） were4.2±0.3,4.00.3, 5.0±0.2andS. 6±0.8 （P 〈 0.01 ）. Comparing the results of blood cell count between control, V1, V2 and P groups, RBC （10^12/L） were 10. 58 ± 0.55, 8.84±0.42, 8.39±0.46 and 7.71 ±0.54 （P〈0.01）, WBC （10^9/L） were 8.24±2.54, 4.32±1.06, 3.57± 0. 75 and 4. 12 ± 0. 80 （P 〈 0. 01 ）, and Plt（109/L） were 724 ± 80, 661 ± 103, 577 ± 66 and 607 ± 94 （P 〉 0.05 ）. Con- clusion The hemodynamic changes of 30% and 40% volume-fixed group are close to expected clinical values, which are suitable for the research on metabolism, pathological physiology, survival condition and treatment strategies after hemorrhagic shock. The 35 -40 mmHg pressure-fixed group display characteristics of severe hemorrhagic shock and smaU blood pressure variability, which makes it suitable to study metabolic acid-base balance disorders, pharmacodynamics, organ damage and molecular mechanism after hemorrhagic shock.