不同干燥方法对白芍中6种化学成分的影响与评价

Effect of Different Drying Methods on Six Chemical Components of Paeoniae Radix Alba

研究不同干燥方法对白芍中单萜苷类和酚酸类化学成分的影响,为白芍适宜干燥方法的确定提供依据;采用HPLC同时测定经不同干燥方法制备的样品中4种单萜苷类、2种酚酸类化学成分的含量;利用SPSS软件进行主成分分析,利用主成分得分进行综合评价。结果表明:不同干燥方法所得白芍均能达到2015年版《中国药典》对白芍含量和水分的要求,采用真空冷冻干燥单萜苷类和酚酸类总量最高,为6.248%,热风60℃干燥次之,为6.141%;经主成分分析,不同干燥方法处理的白芍中单萜苷类和酚酸类成分含量综合评分依次为:真空冷冻干燥>热风60℃干燥>微波干燥>远红外干燥>晒干>热风40℃干燥>热风80℃干燥>阴干>热风100℃干燥。不同干燥方法对白芍中单萜苷类和酚酸类成分有一定的影响。因此,从各成分含量、成本和实用性方面综合分析,热风60℃干燥为白芍适宜的干燥方法。

The aim of this study was to investigate the effects of different drying methods on the contents of monoterpene glycosides and phenolic acids in Paeoniae Radix Alba,and to provide a scientific basis for the selection of appropriate drying method for Paeoniae Radix Alba. HPLC was used to simultaneously determine the contents of 4 monoterpene glycosides and 2 phenolic acids with different drying methods. Principal component analysis（ PCA） was carried out by SPSS software to evaluate the quality of different processed products from Paeoniae Radix Alba. The results showed that the contents of chemical components and water content were in accordance with the requirements of Chinese Pharmacopoeia（ Version 2015）. The highest total contents of monoterpene glycosides and phenolic acids in Paeoniae Radix Alba processed by vacuum freeze drying were 6. 248% and followed by processed by drying with hot air at 60 ℃（ 6. 141%）.According to the PCA results,the comprehensive scores of monoterpene glycoside and phenolic acid compounds in Paeoniae Radix Alba processed with different drying methods in the order from high to low were vacuum freeze dried dried with hot air at 60 ℃ microwave dried far infrared dried sun dried dried with hot air at 40 ℃ dried with hot air at 80 ℃ shade dried dried with hot air at 100 ℃. In conclusion,different drying methods had certain effects on the content monoterpene glycoside and phenolic acid compounds in Paeoniae Radix Alba. Drying with hot air at 60 ℃ was the appropriate drying method of Paeoniae Radix Alba by comprehensive analysis of the content,cost and practicality.