三种方法测定鲨鱼硫酸软骨素钠含量的比较

Comparison of Three Methods for Determination of Chondroitin Sulfate Sodium Shark

利用酶解高效液相色谱法、高效液相色谱法及氯化十六烷基吡啶电位滴定法对鲨鱼硫酸软骨素钠含量进行检测,并对检测结果进行对比分析,结果显示,酶解高效液相色谱法不适用于鲨鱼硫酸软骨素钠含量的测定;而氯化十六烷基吡啶电位滴定法检测鲨鱼硫酸软骨素钠含量稍高于高效液相色谱法;相比之下,高效液相色谱法较适用于检测鲨鱼硫酸软骨素钠含量。

The content of chondroitin sulfate sodium shark（ CS） was separately evaluated by enzymatic-high performance liquid chromatography（ E-HPLC）,high performance liquid chromatography（ HPLC） and cetylpyridinium chloride（ CPC）-titration methods. After comparing and analyzing the detection results,it was found that E-HPLC method was not suitable for determining chondroitin sulfate sodium shark. Moreover,the content determined by CPC-titration method was higher than that of HPLC method. By contrast,HPLC was more suitable for determining of chondroitin sulfate sodium shark.