茜草炭对急性血瘀模型大鼠抗凝血倾向的活性部位筛选

Screening of Effective Parts in Rubiae radix et rhizome Carbonisatum with Resisting Coagulation Tendency in the Acute Blood Stasis Model Rat

筛选茜草炭抗凝血作用有效部位,以急性血瘀模型大鼠为研究对象,云南白药为阳性药,除空白组外,其余各组均ih盐酸肾上腺素加冰水浴复制大鼠急性血瘀模型,考察茜草炭各极性部位对血瘀模型大鼠体外血栓、血液流变学、内/外源性凝血系统、血小板系统以及纤溶系统的影响。结果表明茜草炭正丁醇部位具有较强的抗凝血作用,可显著降低急性血瘀模型大鼠体外血栓干重,中、低切黏度,纤溶酶原激活物抑制物、血栓素含量,升高纤溶酶原激活物、6-酮-前列腺素含量(P<0.05或P<0.01)。其他极性部位对急性血瘀模型大鼠抗凝血作用不及正丁醇部位。结果显示正丁醇部位为茜草炭抗急性血瘀模型大鼠凝血倾向的活性部位。

The goal of this research was to screen of effective parts in Rubiae radix et rhizome Carbonisatum with resisting coagulation tendency. The acute blood stasis model in rats were established with subcutaneous injection of high-dose adrenaline hydrochloride and being socked in ice-water,using Yunnan Baiyao powder as positive drug. The effect of different polar fractions on hemorheology,external thrombus,blood plotelets system and fibrinolytic system in acute blood stasis model rats were observed. The results showed that the n-butanol fraction had the highest effect in resisting coagulation tendency which both significantly decreased the whole blood viscosity in medium and low shear rate,the wet weight and dry weight of external thrombus,significantly decreased the content of PAI-1 and TXB2,increased the t-PA and the6-keto-PGF1αcontent（ P〈0. 05 or P〈0. 01）. The effect of resisting coagulation tendency in other polar parts were lower than that of n-butanol fraction. Hence,the n-butanol fraction were the effective fractions in resisting coagulation tendency.