非小细胞肺癌胸水细胞块c-MET基因扩增检测分析

Analysis of cell blocks c-MET amplification in pleural effusion of non-small cell lung cancer

目的:探讨非小细胞肺癌胸水细胞块在间充质上皮转化因子受体(c-mesenchymal-epithelial transition,c-M ET)基因扩增检测中的临床价值。方法:采用RT-PCR法检测215例非小细胞肺癌细胞块和404例非小细胞肺癌组织块中c-MET基因扩增,并检测细胞块同时送检组织块的患者74例的一致性。结果:细胞块c-MET基因扩增31例,扩增率14.42%(31/215);组织块c-MET基因扩增3 5例,扩增率8.6 6%(3 5/4 0 4);7 4例有组织块对照的细胞块c-M ET结果一致性有6 8例,一致率达9 1.8 9%(6 8/7 4),其中细胞块c-M ET基因扩增率1 2.1 6%(9/7 4),组织块扩增率1 7.5 7%(1 3/7 4)。结论:非小细胞肺癌胸水细胞块c-MET的扩增率略高于组织块;有恶性胸水的非小细胞肺癌患者原发灶组织发生c-MET扩增的概率较高。

Objective： To investigate the clinical value of the cell blocks for gene amplification detection of c-mesenchymal-epithelial transition （c-MET）. Methods： Two hundred and fifteen cases of cell block from pleural effusion of non-small cell lung cancer （NSCLC） were collected. Four hundred and four cases of tissue block were detected by RT-PCR method. The consistency of c-MET amplification was examined in 74 cases of patients with tissue block and cell block. Results： c-MET amplification was found in 31 of 215 cell blocks （positive detection rate of 14.42%）. c-MET amplification was detected in 35 of 404 tissue blocks （positive detection rate of 8.66%）. There were 68 cases in the 74 （91.89%） cases had the same consistency as tissue block. c-MET amplification was detected in 9 of 74 （12.16%） cell blocks, and 13 of 74 （17.57%） tissue blocks. Conclusion： The rate of c-MET amplification in cell blocks of non-small cell lung cancer is higher than in matched tissue blocks. The patients with malignant pleural effusion are likely to tend c-MET amplification.