乙肝肝硬化致肝癌患者癌组织miRNA-145的表达及其与预后的关系

Expression of miRNA-145 in Patients with Liver Cancer Induced by Hepatitis B Liver Cirrhosis and Its Relationship with Prognosis

【目的】探析乙肝肝硬化致肝癌患者癌组织miRNA-145的表达情况及与预后的关系。【方法】选取本院2009年1月至2012年1月共收治的74例乙肝肝硬化致肝癌患者作为研究对象，均于术中留取癌组织标本，进行癌组织中miRNA-145表达的检测及实时定量RT-PCR、细胞增殖检测及侵袭试验，根据miRNA-145浓度分为50nmol／L、100nmol／L、150nmol／L，对照组为体脂质体处理。结合临床探讨乙肝肝硬化致肝癌患者的miR—NA-145表达、miRNA-145对肿瘤细胞增殖能力、侵袭与转移能力的影响及与患者预后的关系。【结果】肝癌组织与人肝癌HepG2细胞株的miRNA-145表达水平均高于正常组织，差异有统计学意义（P〈0．05）。24h时各组细胞相对增殖（D490）的差异无统计学意义（P〉0．05）；48h与72h时，miRNA-145转染不同浓度组的细胞相对增殖（D490）均低于脂质体处理组，差异有统计学意义（P〈0．05）。试验组相对穿出小室膜的肿瘤细胞数为（0．29±0．05）个，低于脂质体处理组的（0．36±0．12）个，差异有统计学意义（P〈0．05）。miRNA-145高表达患者的生存期长于低表达组，差异有统计学意义（P〈0．05）。【结论】miRNA-145的表达可抑制肿瘤增殖、控制肿瘤进展，并减弱肿瘤细胞的侵袭与转移能力、促进肿瘤细胞凋亡，从而延长肝癌患者生存期，值得临床深入研究。

[Objective]To study the expression of cancer tissue miRNA-145 in patients with liver cancer induced by Hepatitis B liver cirrhosis and its relationship with prognosis.[Methods]Seventy-four patients with liver cancer induced by hepatitis B cirrhosis who were admitted in our hospital between January 2009 and January 2012 were selected as the study objects. Cancer tissue specimens were collected from all patients during operation. The expression of miRNA-145 in tumor tissues, the real-time quantitative RT-PCR and cell proliferation were detec- ted, and cell invasion assay was performed. According to miRNA-145 concentration , 50 nmol/L, 100 nmol/L and 150 nmol/L were sub-divided , and the control group was treated with liposome. The effect of miRNA-145 expression and its ability on proliferation of tumor cells, the impact of invasion and metastasis in patients with liver cancer induced by Hepatitis B liver cirrhosis and their relationships with prognosis were investigated and concluded. [Results]The expression levels of cancer tissues and miRNA-145 in Human hepatocellular carcinoma HepG2 cell line were higher than those in normal tissues, the difference was statistically significant （ P 〈0.05）. There was no significant difference in the relative proliferation （D490） in each group in 24 h （ P 〉0.05） ; in 48h and 72h, the cell relative proliferation （D490） of miRNA-145 transfection in different concentration groups was lower than that in the liposome treated group, and the difference was statistically significant （ P 〈0.05）. The number of tumor ceils through the cell membrane of the experimental group was （0.29±0.05）, relatively lower than that of the liposome treated group（0.36±0.12）, the difference was statistically significant （ P 〈0.05）. The survival time of patients with higher expression of miRNA-145 was longer than that in the lower expression group, and the difference was statistically significant （ P 〈0.05）.[Conclusion]The expression of miRNA145 can inhibit tumor proliferation, control tumor progression, reduce invasion and metastasis of tumor cells, and promote apoptosis of tumor cells, thus it is able to prolong the survival time of patients with liver cancer. It is worth clinical in-depth study.