血管内皮细胞活化在感染后肠易激综合征发病机制中的作用

Role of vascular endothelial cell activation in the pathogenesis of post infectious irritable bowel syndrome

目的研究感染后肠易激综合征(PI-IBS)患者中肠血管内皮细胞形态以及血浆中内皮素(ET)和一氧化氮(NO)表达水平的变化,明确血管内皮细胞活化在PI-IBS发病机制中的作用。方法同期收集就诊的PI-IBS患者28例和正常对照组12例,采集所有研究对象的空腹血标本和结肠标本。用光学显微镜观察内皮细胞形态和变化,用放射免疫法和硝酸还原法分别检测血浆中ET和NO表达水平的变化。结果 PI-IBS患者内皮细胞形态较正常对照组发生显著变化,功能上的变化表现为血浆中ET(55.49±13.49)ng/L和NO(75.90±16.52)μmol/L表达水平较正常对照组[(43.40±13.69)ng/L和(62.48±15.63)μmol/L]明显增高(P<0.05)。结论血管内皮细胞活化与PI-IBS的发病机制可能密切相关。

Objective To investigate the morphological changes of vascular endothelial cells as well as changes in the level of plasma endothelin（ ET） and nitric oxide（ NO） in patients with post infectious irritable bowel syndrome（ PIIBS）,in order to clarify the possible role of vascular endothelial cell activation in the pathogenesis of PI-IBS. Methods The mucosa biopsy samples and plasma were obtained from 28 PI-IBS patients and 12 healthy controls during the same period. The morphological changes of vascular endothelial cells were observed with light microscope. The plasma ET and NO levels in both groups were measured with radioimmunoassay and nitrate reductase method. Results There were more significant morphological changes in vascular endothelial cells in the PI-IBS patients than in the controls. The plasma ET and NO levels of the PI-IBS patients were（ 55. 49 ± 13. 49） ng / L and（ 75. 90 ± 16. 52） μmol / L,respectively,which were significantly higher than those of the controls[（ 43. 40 ± 13. 69） ng / L and（ 62. 48 ± 15. 63） μmol / L,both P〈0. 05]. Conclusion The vascular endothelial cell activation may be closely related to the pathogenesis of PI-IBS.