里氏木霉纤维素酶水解稻草的研究

Enzymatic Hydrolysis of Rice Straw by Trichoderma reesei Cellulase

[目的]探讨碳氮源对里氏木霉发酵产纤维素酶的影响,以及纤维素酶水解稻草的条件。[方法]通过添加不同的碳源和不同浓度的酵母粉,探讨里氏木霉合适的发酵条件;使用不同添加量的纤维素酶对稻草进行酶解;分别利用纤维素酶、纤维素酶和木聚糖酶混合酶对稻草进行酶解反应。[结果]利用乳糖和稻草的复合碳源和12 g/L的酵母粉进行水解时,纤维素酶活性较高。酶解适宜的酶用量为每克稻草底物200 U的滤纸酶。用纤维素酶及木聚糖酶混合酶酶解稻草96 h的酶解得率为65.4%。[结论]该研究可为里氏木霉纤维素酶生产和酶解稻草的应用提供一定的依据。

[Objective] To investigate the effects of carbon and nitrogen sources on cellulase production by Trichoderma reesei and the conditions for enzymatic hydrolysis of rice straw. [Method] The fermentation conditions of T. reesei were studied by adding different carbon sources and yeast powder of different concentration. Enzymatic hydrolysis of rice straw was carried out using different amounts of cellulase,and the enzymatic hydrolysis reaction of rice straw was carried out by cellulase and mixed enzyme of cellulase and xylanase. [Result] Cellulase activity was high when the compound carbon source of lactose and rice straw and yeast powder concentration of 12 g / L were used. The suitable amount of enzyme was 200 U（ FPA） per gram of rice straw for enzymatic hydrolysis. After 96 h enzymatic hydrolysis of rice straw,the yield of 65. 4%was achieved by combination of cellulase and xylanase. [Conclusion] The study provided a certain basis of application of T. reesei cellulase production and enzymatic hydrolysis of rice straw.