烟草bZIP转录因子BZI-4的生物信息分析

Biological Information Analysis of b ZIP Transcription Factor BZI-4 in Tobacco

[目的]探讨烟草在采收前的生理期和烘烤过程中的变黄阶段抗逆机制。[方法]利用生物信息学方法对烟草b ZIP转录因子BZI-4的理化特性、信号肽、跨膜、系统进化和亚细胞定位进行分析。[结果]烟草b ZIP转录因子BZI-4为碱性、水溶性、耐高温和非分泌型的游离蛋白,该蛋白为叶绿体中的转运肽,仅剪切修复后才具有活性,对环境适应能力强。[结论]通过烟草b ZIP转录因子BZI-4研究,为烟草抗逆境胁迫研究提供理论基础。

[Objective]To discuss the related factors of resistance to stress in the physiological period of tobacco before harvesting and baking yellowing stage. [Method] The physical and chemical properties,signal peptide,transmembrane,system evolution and subcellular localization of tobacco b ZIP transcription factor BZI-4 were analyzed by bioinformatics methods. [Result] Tobacco b ZIP transcription factor bzi-4 was alkaline water solubility and relative stability,high heat resistance,non-secretion type of free protein. The protein might be existed in chloroplast transit peptide,its activity must be exerted through the amino acid sequence of shear,and the condition of adaptive evolution was moderate. [Conclusion] The study of b ZIP transcription factor BZI-4 in tobacco provides a theoretical basis for the study of tobacco resistance to stress.