山茱萸和枸杞乙醇提取物抗氧化活性研究

Study on the Antioxidant Activity of Fructus Corni and Fructus Lycii Ethanol Extracts

主要研究山茱萸和枸杞的多酚含量及乙醇提取物的抗氧化活性。用60%乙醇30℃条件下分别浸提山茱萸和枸杞干果,分别得到山茱萸和枸杞乙醇提取物;利用FC法测定多酚含量,FRAP和DPPH法测定提取物抗氧化活性。结果显示山茱萸多酚含量为14.33±0.50mg/g,山茱萸乙醇提取物FRAP值为0.720±0.030mmol/g,DPPH·半数清除浓度(IC50)为1.504±0.012 mg/mL;枸杞多酚含量为12.53±0.62 mg/g,枸杞乙醇提取物FRAP值为0.630±0.042mmol/g,DPPH·半数清除浓度(IC50)为2.214±0.023mg/mL。山茱萸与枸杞乙醇提取物均有较好的抗氧化活性,清除DPPH·的能力优于维生素C。

Polyphenol content and antioxidant activity of ethanol extracts of Fructus corni and Fructus lycii were studied in this article.Ethanol extracts were obtained from Fructus corni and Fructus lycii with 60% ethanol and 30 ℃ temperature respectively,polyphenol contents were determined on the FC method and antioxidant activity on FRAP and DPPH.The results showed that polyphenol contents（14.33±0.50mg/g,12.53±0.62mg/g）,FRAP values of ethanol extract（0.720±0.030mmol/g,0.630± 0.042 mmol/g）and IC50 of DPPH·（1.504 ± 0.012mg/mL,2.214±0.023mg/mL）were discovered from Fructus corni and Fructus lycii respectively.Ethanol extracts of Fructus corni and Fructus lycii both showed a good antioxidant activity and the inhibitory ability for DPPH· was better than vitamin C.