不同分子量八珍汤水溶多糖的分离和活性研究

The separation of different molecular weight polysaccharide from Bazhen decoction and analysis of their bioactivity

目的：提取并分离不同分子量八珍汤水溶多糖，并研究其活性。方法水提醇沉法提取八珍汤水溶性粗多糖，采用滤膜离心法将多糖按分子量分为3段，低、中和高分子量分别标记为Ⅰ、Ⅱ和Ⅲ段，其分子量分别为≤3×10^4，3×10^4～10^5和≥105。采用流式细胞术分析多糖对细胞活力、活性氧（ ROS）及细胞周期的影响。结果3种不同分子量多糖对K562细胞活力无影响，但Ⅰ和Ⅲ段多糖可增加K562和THP-1细胞内活性氧，并促进THP-1细胞增殖。结论不同分子量的八珍汤多糖对白血病细胞活力无显著影响，但低和高分子量多糖可增加髓系来源细胞（ K562和THP-1） ROS水平，并促进THP-1细胞增殖。

Objective To separate different molecular weight polysaccharide from Bazhen decoction and analyse their bioactivity .Methods The crude polysaccharide of Bazhen decoction was obtained by aqueous extraction and alcohol precipitation .The crude polysaccharide was separated into 3 groups by different molecular weight using centrifuge through filter membrane,including group Ⅰby less than 3 ×10^4,group Ⅱ by 3 ×10^4 to 10^5,and group Ⅲ by more than 10^5 .The ROS level,cell viability and cell cycle were analysed by flow cytometry .Results The cell viability of K562 leukemic cells was not affacted by all the three groups polysaccharide .But it was found that groups Ⅰ and Ⅱpolysaccharide could evoked ROS generation in K 562 cells and macrophage THP-1 cells.It was also confirmed that groups Ⅰand Ⅱpolysaccharide could promote THP-1 cell proliferation .Conclusion Different groups of polysac-charide from Bazhen decoction has no effect on the viability of K 562 cells.But the higher and lower molecular weight polysaccharide could provoked ROS in myelogenous cells （ K562 and THP-1） and promoted the proliferation of macro-phage THP-1 cells.