水迷宫法测定补骨脂素对去卵巢痴呆大鼠行为学的影响

The Effects Analysis of Psoralen on Praxiology of Ovariectomized Rats with Dementia by Water Maze Test

目的:探讨水迷宫法测定补骨脂素对去卵巢痴呆(AD)大鼠行为学的影响。方法:于2015年1月至2015年12月,选择健康雌性Wistar大鼠30只,随机分成对照组、模型组、补骨脂素组,每组10只;模型组、补骨脂素组按常规建立去卵巢痴呆模型,3组都给予常规喂养,卵巢切除术后2周,补骨脂素组大鼠灌胃,对照组和模型组给予1.5 mL/d的0.9%氯化钠注射液灌胃。结果:造模后模型组、补骨脂素组与造模前比较,逃避潜伏期时间明显延长(P<0.05),跨越平台次数明显减少(P<0.05);补充补骨脂素治疗后,补骨脂素组大鼠逃避潜伏期明显缩短,跨越平台次数增加,与模型组比较差异具有统计学意义(P<0.05)。结论:补骨脂素有效预防去卵巢AD对大鼠记忆功能的损伤,可弥补去卵巢对大鼠智能的损害。

Objective To investigate the effects of psoralen on praxiology of ovariectomized rats with dementia by water maze test.Methods The time study were from January2015 to December 2015,30 healthy female Wistar rats were chosen and randomly divided into control group,model group,Fructus Psoraleae group,with 10 rats in each group.The model group,psoralen group were routinely to establish a model of ovarian dementia.Three groups were given routine feeding,after ovariectomy 2 W,psoralen group rat intragastric administration,the control group and model group were given 1.5 ml saline gavage.Results After modeled,the escape latency were significantly prolonged in the model group and Fructus Psoraleae group,（P0.05）,and across the platform number significantly decreased（P0.05）; and the psoralen treatment of psoralen group rats escape latency were significantly shorter,cross platform number were increased,that compared to the model group were significant difference（P0.05）.ConclusionPsoralen can effective prevent damage the memory function of rats ovarian dementia,it can compensate for the damage of the ovary of rat intelligence that has good application value.