HPLC法同时测定十味玉泉片中4种有效成分

Simultaneous Determination of Four Active Components in the Shiwei Yuquan Tablets by HPLC

目的:建立高效液相色谱法同时测定十味玉泉片中葛根素、甘草苷、甘草酸铵和五味子醇甲4种有效成分的含量。方法:色谱柱为ZORBAX SB-C18柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸水溶液进行梯度洗脱;流速为1.0 m L·min^(-1);柱温为30℃;检测波长(0~8 min在250 nm波长下检测葛根素,8~14 min在276 nm波长下检测甘草苷,14~25 min在250 nm波长下检测甘草酸铵,25~40min在216 nm波长下检测五味子醇甲)。结果:4个有效成分的线性范围分别为葛根素68.05~680.5μg·m L^(-1)(r=0.999 9)、甘草苷22.06~220.6μg·m L~(^(-1))(r=0.999 8)、甘草酸铵24.98~249.8μg·m L^(-1)(r=0.999 4)、五味子醇甲2.184~21.84μg·m L^(-1)(r=0.998 9),平均加样回收率分别为98.83%、97.84%、98.09%、97.20%,RSD分别为0.71%、0.69%、0.80%、0.99%(n=6)。结论:该方法简便、准确、灵敏度高、重复性好,可用于同时测定十味玉泉片中上述4个有效成分的定量测定,控制产品质量。

Objective： To establish an HPLC method for simultaneous determination of four active components（ puerarin; liquiritin; ammonium glycyrrhetate; schisandrin） in the Shiwei Yuquan tablets. Methods： Zorbax SB-C18（ 250 mm × 4. 6 mm,5 μm） column was used with mobile phase consisting of water containing 0. 1% phosphoric acid and acetonitrile in gradient mode. The flow rate was 1. 0 m L·min^-1,The column temperature was 30 ℃. Puerarin was detected at250 nm,liquiritin was detected at 276 nm,ammonium glycyrrhetate were detected at 250 nm,and schisandrin was detected at216 nm. Results： There was good linear relationship between the concentration of puerarin, liquiritin, ammonium glycyrrhetate,schisandrin,and peak area within the concentration range of 68. 05- 680. 5 μg·m L^-1（ r = 0. 999 9）,22. 06-220. 6 μg·m L^-1（ r = 0. 999 8）,24. 98- 249. 8 μg·m L^-1（ r = 0. 999 4）,2. 184- 21. 84 μg·m L^-1（ r = 0. 998 9）. The average recovery（ RSD） was 98. 83%（ 0. 71%）,97. 84%（ 0. 69%）,98. 09%（ 0. 80%）,97. 20%（ 0. 99%）,respectively（ n = 6）. Conclusion： The established method is accurate,highly sensitive and well reproducible,which can be used for the determination of the four active ingredient in the Shiwei Yuquan tablets,control product quality.