桔梗对甘草中抗炎成分提取的影响

The Effect of Platycodon Grandiflorum on the Anti-inflammatory Components in Licorice Extract

通过对比甘草单煎以及与桔梗共煎后抗炎成分含量的变化探究甘草桔梗的配伍机理。采用高效液相色谱法对甘草提取液以及甘草-桔梗提取液中的抗炎成分主要是甘草酸以及甘草次酸进行定量分析;甘草酸在0.02~2 mg/m L浓度内保持较好的线性关系;甘草次酸在0.02~1 mg/m L浓度内保持较好的线性关系。重复性实验甘草酸RSD为1.30%（n=4）,甘草次酸RSD为1.41%（n=4）;精密度实验甘草酸RSD为2.00%（n=5）,甘草次酸RSD为1.73%（n=5）;稳定性实验甘草酸RSD为1.32%（n=4）,甘草次酸RSD为0.85%（n=4）,供试液中的甘草酸以及甘草次酸能够在9 h内保持稳定;回收率实验中甘草酸回收率等于96.96%（n=3）,甘草次酸回收率等于106.41%（n=3）。相比于甘草单煎,加入桔梗后,同数量的甘草通过煎煮,增加了抗炎物质的含量,从而提升了该方剂的疗效。

By contrast with bellflower licorice decocted,the anti-inflammatory ingredient content changes to explore the compatibility of the mechanism of licorice bellflower. High performance liquid chromatography（ HPLC） were used for licorice extract and licorice-bellflower extract to proceed quantitative analysis of anti-inflammatory components; the column is SymmetryC18column,the mobile phase was methanol-water gradient elution,detection wavelength of 254 nm. Glycyrrhizinate at 0. 02-2 mg / m L range with good linearity; glycyrrhetinic acid in the 0. 02-1 mg / m L range with good linearity. Repeatability Glycyrrhizinate RSD is 1. 30%（ n = 4）,glycyrrhetinic acid RSD is 1. 41%（ n = 4）,good repeatability; Glycyrrhizinate precision RSD is 2. 00%（ n = 5）,glycyrrhetinic acid RSD is 1. 73%（ n = 5）; stability testing glycyrrhizinate RSD is 1. 32%（ n = 4）,glycyrrhetinic acid RSD is 0. 85%（ n = 4）,licorice and glycyrrhetinic acid stable in 9 h; recovery experimental glycyrrhizinate recovery is 96. 96%（ n = 2）,glycyrrhetinic acid recovery is106. 41%（ n = 2）. Licorice-bellflower measured by HPLC method in anti-inflammatory substances have high specificity,high precision,good repeatability,and the operation is simple,convenient,that method is a simple and reliable way for quantitative analysis. Data obtained by this method can be oriented subject conclusions which provide a scientific basis. Compared alone decocted in licorice,licorice decoction of adding bellflower,with same quantity has increased content of anti-inflammatory substances,thereby enhancing the efficacy of the prescription.