水通道蛋白磁共振分子成像与水通道蛋白4表达的相关性研究

Research on correlation between aquaporin magnetic resonance molecular imaging and AQP4 expression

目的：探讨水通道蛋白磁共振分子成像（AQPMRI）与水通道蛋白4（AQP4）表达量的相关性。方法：10只成年雄性SD大鼠随机分为两组，分别经单纯的脑缺血模型及肢体远端缺血（LRP）预处理（LRP组）或阴性预处理（Stroke组）后经历1h的大脑中动脉栓塞建立脑缺血模型。在恢复再灌注后48h行MRI检查采集AQPMRI图像，并行Western blot检测AQP4表达量。结果：10只大鼠最终符合建模成功条件的为6只，建模成功率为60％。AQPMRI显示1～5号大鼠患侧的AQPADC值较健侧降低，而6号大鼠患侧的AQPADC值较健侧显著升高。Western blot结果显示1-6号大鼠患侧的AQP4表达量较健侧升高。相关性分析显示，将6号大鼠剔除后，1～5号大鼠的AQPADC值与AQP4表达量呈负相关。结论：AQPMRI能够在体显示AQP4的表达量及分布情况，具有广阔的临床应用价值。

Objective： To explore the correlation between aquaporin magnetic resonance molecular imaging （AQP MRI） and the expression of AQP4. Methods： Ten adult SD male rats were subjected to either predisposing （LRP） or sham surgery and then subsequently underwent fight middle cerebral artery occlusion （MCAo） for one hour. AQP MRI was executed 48 h after reperfusion. And the AQP4 expression was detected by Western blot. Results： The success rate of modeling was 60%, meaning there were only 6 rats living up to the criteria of modeling. AQP MRI showed AQP ADC values of the affected side of rats No.1-5 were lower than those of the uninjured side, but the AQP ADC values in the affected side of rat No.6 was much higher than that of the uninjured side. Western blot results showed that the AQP4 expression in the affected side of No.1-6 rats was higher than that of the uninjured side. Correlation analysis showed that the AQP ADC values were negatively correlated to the level of AQP4 protein expression when excluding rat No.6. Conclusion： AQP MRI can display the expression quantity as well as the distribution of AQP4 in vivo, and it has great value in clinical application.