HLA-DQB1 基因与中国湖北汉族人 SLE 及其自身抗体的相关性研究

ASSOCIATION OF HLADQB1 ALLELE WITH THE SUSCEPTIBILITY TO SYSTEMIC LUPUS ERYTHEMATOSUS AND AUTOANTIBODIES IN CHINESE HUBEI HAN

目的：探讨ＨＬＡＤＱＢ１等位基因与系统性红斑狼疮（ＳＬＥ）及其自身抗体的相关性。方法：采用ＰＣＲ／ＳＳＰ技术对５２例中国湖北地区汉族ＳＬＥ患者及１４３例正常对照者进行了ＨＬＡＤＱＢ１基因分型，并采用免疫印迹技术检测患者血清中自身抗体。结果：ＳＬＥ患者ＤＱＢ１０６０８（９６２％，χ２＝１０５１，Ｐ＜０００５）基因频率显著升高，ＤＱＢ１０３０２（５７７％，ＲＲ＝０２６Ｐ＜００５，ＰＦ＝０１４）和ＤＱＢ１０５０１（１９２％，ＲＲ＝０１１，Ｐ＜００１，ＰＦ＝０１３）基因频率显著降低，与正常对照组比较，ＤＱＢ１０６０８在伴抗Ｓｍ（１０３４％，Ｐ＜０００５）、抗ＲＮＰ（１１５４％，Ｐ＜０００５）、抗ｄｓＤＮＡ（２２２２％，Ｐ＜０００５）抗体阳性的ＳＬＥ患者中频率显著升高。结论：ＤＱＢ１０６０８与ＳＬＥ关联，并分别与抗Ｓｍ、抗ＲＮＰ、抗ｄｓＤＮＡ抗体的产生有相关性。而ＤＱＢ１０３０２、ＤＱＢ１０５０１等位基因对ＳＬＥ可能具有保护性。

Objective:To study the association of HLADQB1 allele with SLE and autoantibodies in Chinese Hubei Han NationalityMethods:Fiftytwo patients of Chinese Hubei Han Nationality with systemic lupus erythematosus (SLE) for HLADQB1 gene analysis by PCR/SSP technique and using immunoblotting for measure of autoantibodies.Results:Compared with normal controls, subjects with SLE showed an increased frequency of HLADQB1*0608(962%,χ2=1051,P<0005)and decreased frequencies of HLADQB1*0302(577%,RR=026,P<005,PF=014) and DQB1*0501(192%,RR=011,P<001,PF=013).The frequency of HLADQB1*0608 was increased in patients with antiSm(1034%,P<0005),antiRNP(1154%,P<0005) and antidsDNA(2222%,P<0005) autoantibodies compared with normal controls Conclusion: These results suggest that susceptibility to SLE is strongly associated with the HLADQB1*0608 allele。The HLADQB1*0608 allele is strongly associated with antiSm antiRNP and antidsDNA autoantibodies in patients with SLE