天蓝苜蓿黄酮组分的制备及清除DPPH自由基活性研究

The Preparation of Flavonoid Components from Medicago Lupulina L. and the Free of DPPH Radical Scavenging Activities

目的:以聚酰胺色谱柱分离天蓝苜蓿中黄酮组分,研究其清除DPPH自由基的能力。方法:采用多功能动态热回流提取浓缩机组提取天蓝苜蓿乙醇提取物,通过聚酰胺色谱柱吸附黄酮成分,不同浓度乙醇洗脱后,干燥得不同浓度乙醇洗脱物;采用DPPH自由基清除法,比较天蓝苜蓿不同洗脱液中黄酮组分的抗氧化能力。结果:天蓝苜蓿黄酮以三氯化铝显色后于紫外423nm有最大吸收;聚酰胺色谱柱上样洗脱,上样速度:12m L/min,洗脱速度:18m L/min;不同溶剂洗脱物清除DPPH自由基能力为:30%乙醇洗脱物>60%乙醇洗脱物>90%乙醇洗脱物>水洗脱物。结论:聚酰胺色谱柱能够富集分离天蓝苜蓿黄酮组分,且30%乙醇洗脱物清除DPPH自由基能力最强。

Objective By polyamide chromatographic column separation of flavonoid components from Medicago lupulina L. and investigate the DPPH radical scavenging ability. Methods Multifunctional dynamic heat reflux extraction and concentration unit was used to extract the ethanol extract from Medicago lupulina L. and absorbed by polyamide chromatographic column, The column was eluted by ethanol with different concentration. The effluents were evaporated and dried in Vacuum, respectively. The antioxidant proper- ties of different effluents were undertaken employing DPPH scavenging activity. Results Confirm that after chemical coloration of alchlor, by ultraviolet spectrophotometer at 423 nm measured Medicago lupulina L. Sample elution of polyamide chromatographic column, sample rate ： 12mL/min, elution rate ： 18mL/min. The ability of scavenging DPPH free radical with different solution was ： 30% ethanol elution 〉 60% ethanol elution 〉 90% ethanol elution 〉 queous elution. Conclusion Polyamide chromatographic column can be used to the enrichment and purification of flavonoids from Medicago lupulina L. 30% ethanol elution showed best DPPH radical scavenging ability than other parts.