摘要
Ipomoea trifida(Kunth)G.Don(2n=2x=30)是甘薯祖先种之一,具有较强的抗旱性,其抗旱生理机制尚无报道。本文采用营养液水培法,以30%浓度的PEG6000模拟干旱,研究干旱胁迫对甘薯近缘野生种幼苗叶片生理生化指标的影响。结果表明:随着PEG6000干旱胁迫时间的延长,叶片中丙二醛(MDA)、游离脯氨酸(Pro)含量逐渐增加。过氧化物酶(POD)和过氧化氢酶(CAT)及谷胱甘肽还原酶(GR)活性在PEG处理后活性均增强,揭示了甘薯近缘野生种MDA和Pro含量和氧化酶类的活性在短时间急性胁迫情况下的不同变化规律,可为抗旱品种的筛选和下一步抗旱相关基因的挖掘提供一定的参考。
Ipomoea trifida（Kunth） G.Don（2n = 2x = 30） was one of the ancestors of a sweetpotato and had high-drought resistance,however,its drought-resistant physiological mechanism was still not reported.In this study,using the method of the simulated-drought water culture with 30 % concentrations of PEG-6000,the effects of drought stress on the physiological and biochemical indexes of sweetpotato seedling leaf of wild relatives were studied.The results showed that the malonaldehyde（MDA） and free proline（Pro） content increased with the prolongation of PEG-6000 treatment time.Peroxidase（POD）,catalase（CAT） and glutathione reductase（GR） activity were enhanced after PEG treatment,which revealed different changing law of the MDA and Pro content of sweetpotato wild relatives and their enzyme activity in a short period of acute stress,which could provide some references for screening drought resistant varieties and tapping drought-resistant related genes.
出处
《西南农业学报》
CSCD
北大核心
2016年第11期2536-2541,共6页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金(31461143017)
江苏省自然科学基金(BK20141144)
江苏省科技支撑计划(BE2014311)
"863"计划(2012AA101204-1-8)
"948"项目(2011-G1-20)
徐州市国际合作项目(XM13B022)
