摘要
将来自于多粘芽孢杆菌(Paenibacillus polymyxa)中的2,3-丁二醇脱氢酶基因pla C在大肠杆菌(Escherichia coli)BL21中进行了诱导表达,并对其酶学性质进行研究。结果表明,该基因的表达产物主要是生成内消旋型2,3-丁二醇,其氧化2,3-丁二醇的最适温度是50℃,最适pH为10,而还原乙偶姻的最适温度为60℃,最适pH为6。
The gene pla C which was in charge of converting acetoin to meso-2,3-butanediol was cloned from Paenibacillus polymyxa. It was expressed in Escherichia coli and subsequently purified. The enzyme activity showed that pla C mainly catalyze meso-2,3-butanediol formation from acetoin. The effects of temperature and pH on the activity of pla C were investigated by using both ketone reduction and diol oxidation reactions. Maximum activity for diol oxidation of pla C was observed at 50℃ and pH 10. The optimal temperature and pH for ketone reduction of pla C were 60 ℃ and pH 6.
出处
《湖北农业科学》
2016年第16期4172-4175,共4页
Hubei Agricultural Sciences
基金
湖北省农业科技创新中心项目(2013NKYJJ21)
国家科技支撑计划项目(2011BAD40B01)
