摘要
目的：建立高效液相色谱（HPLC）测定盐酸托莫西汀含量的方法。方法采用氰基键合硅胶色谱柱（Eclipse XDB-CN，5μm，250 mm＆#215;4.6 mm），以磷酸缓冲液-甲醇（46：54）为流动相，等度洗脱，流速1.0 mL/min，检测波长210 nm。结果线性范围为40.032-60.048μg/mL，r=0.9999；回收率为99.63%-100.80%，总体RSD=0.37%（n=9）。结论本方法简单、迅速、可靠，可用于盐酸托莫西汀的检测和质量控制。
Objective To establish an HPLC method for the determination of atomoxetine hydrochloride.Methods The cyano bonded silica gel column （Eclipse XDB-CN, 5μm, 250 mm×4.6 mm） was used. The isocratic elution was adopted with the mobile phase composed of PBS and methanol （46：54）. The flow rate was 1.0 mL/min and the detective wavelength was 210 nm.Results The linear range of atomoxetine hydrochloride was 40.032-60.048μg/mL and the regression coefficient was 0.9999. The recovery was 99.63 %-100.80 % withRSD of 0.37 % （n=9）. Conclusion The method can be simple, quick and reliable. It can be used for the detection and quality control of atomoxetine hydrochloride.
出处
《食品与药品》
CAS
2016年第6期417-420,共4页
Food and Drug
关键词
高效液相
盐酸托莫西汀
含量
high performance liquid chromatography （HPLC）
atomoxetine hydrochloride
content
