生防菌解淀粉芽孢杆菌KL-1的生物安全性检测

Biological Safety Detection of Antagonistic Bacteria Bacillus Amyloliquefaciens KL-1

为了检测解淀粉芽孢杆菌KL-1菌株的生物安全性,以小鼠为对象,经口急性毒性试验-固定剂量法、染色体畸变分析法、骨髓细胞微核分析法和巨噬细胞吞噬功能分析法评价解淀粉芽孢杆菌KL-1对哺乳动物的安全性.结果表明,14 d的观察中,急性毒性试验中各组小鼠未出现明显中毒症状和死亡情况,且解淀粉芽孢杆菌KL-1对小鼠的半数致死量(LD50)超过2000 mg/kg.染色体畸变分析、骨髓细胞微核分析和巨噬细胞吞噬功能分析结果均为阴性.由此可推断解淀粉芽孢杆菌KL-1菌株对哺乳动物安全无毒副作用,为该菌株作为生物农药的安全使用提供依据.

To detect the biological safety of Bacillus amyloliquefaciens KL-1 , acute oral toxicity-fixed dose proce-dure, chromosomal aberration, bone marrow cell micronucleus and phagocytic function of macrophage tests were carried out in mice. The results showed that there were no significant signs of toxicity and mortality in acute toxicity study in 14 days, and the oral lethal dose of Bacillus amyloliquefaciens KL-1 was more than 2000 mg/kg. The re-sults of chromosomal aberration test, bone marrow cell micronucleus test and macrophage phagocytic function test were all negative. Therefore, it is concluded that Bacillus amyloliquefaciens KL-1 has no toxic and side effects on mammals, and this provides the evidence for the safe use of this strain as a biological pesticide.