中华猕猴桃根乙酸乙酯部分化学成分研究

Study on Chemical Constituents in Ethyl Acetate Extracts from the Root of Actinidia chinensis

对中华猕猴桃(Actinidia chinensis Planch.)根乙酸乙酯部分的化学成分进行研究,依次用石油醚、乙酸乙酯、正丁醇萃取中华猕猴桃根的醇提取物,应用多种色谱技术对乙酸乙酯部分进行分离和纯化,通过波谱数据分析(MS,1H-NMR,13C-NMR)进行结构鉴定。结果表明,从乙酸乙酯萃取部分分离得到6个化合物,经鉴定为2α,24-二羟基乌苏酸、Jacoumaric acid、2α,3α,23-三羟基-12-烯-28-乌苏酸、2α,3α,23-三羟基-12,20(30)-二烯-28-乌苏酸、3β,6α-二羟基豆甾烷、SitoindosideⅠ。其中化合物1、2、5、6首次从该植物中分离鉴定。

To study the chemical constituents from the root of Actinidia chinensis. The root of Actinidia chinensis was extracted with 95% Et OH. The Et OH extract was suspended in H2 O and extracted with petroleum ether,Et OAc and Bu OH successively. The compounds were isolated with column chromatography from the Et OAc fraction,and elucidated on the basis of spectral analyses（MS,1H-NMR,13C-NMR）. The results showed that six compounds were isolated from the root of Actinidia chinensis,and the structures were identified as 2α,24-dihydroxyursolic acid,Jacoumaric acid,2α,3α,23-trihydroxyurs-12-en-28-oic acid,2α,3α,23-trihydroxyurs-12,20（30）-dien-28-oic acid, Stigmastane-3β,6α-diol,SitoindosideⅠ. Compounds 1、2、5and 6 were obtained from the root of Actinidia chinensis for the first time.