摘要
从豆豉中分离到1株产γ-聚谷氨酸(γ-polyglutamic acid,γ-PGA)的菌株JG,通过菌株形态特征和16S rDNA序列对比分析,对该菌株进行了鉴定。同时对发酵产物进行了提纯,并添加不同比例聚谷氨酸到复合肥中,进行盆栽试验。结果表明,该菌株16S rDNA序列与Bacillus subtilis HQ 327126的同源性达到99%,确定该菌株为枯草芽孢杆菌(Bacillus subtilis)。该菌株γ-PGA的发酵产量为40 g/L,对发酵产物进行提纯,获得含量为80%的γ-PGA。添加不同浓度γ-PGA的处理,芜青油菜叶绿素含量明显提高,添加0.02%-0.03%的γ-PGA,芜青油菜鲜重增加可达18.35%-19.57%,具有明显提高肥效的作用。
Abatract: One γ-polyglutamic acid(γ-PGA) producing strain JG was isolated using the conventional methods. The strain was identified through morphological and sequence contrastive analysis of 16 S rDNA. The fermenting product was purified and added into the compound fertilizer by different proportion to carry on the pot planting experiment. The results showed that,the homelogy of 16 S rDNA Sequence of this strain with Bacillus subtilis(HQ327126) reached 99%,thus strain JG was identified as Bacillus subtilis. The yield of γ-PGA was 40 g / L. The fermenting product was purified,and γ-PGA content of 80% was obtained. The adding of γ-PGA has the best effect on increasing the chlorophyll content of Brassica chinensis. Addition of0.02%-0.03% γ-PGA,fresh weight of Brassica chinensis increased by 18.35%-19.57%. γ-PGA added to compound fertilizer could obviously improve the efficiency of the role.
出处
《湖北农业科学》
2016年第17期4569-4571,4576,共4页
Hubei Agricultural Sciences
基金
山东省科技重大专项(新兴产业)(2015ZDXX0502B02)
关键词
Γ-聚谷氨酸
枯草芽孢杆菌
芜青油菜
肥料增效
γ-polyglutamic
Bacillus subtilis
Brassica chinensis
fertilizer efficiency