白藜芦醇对去势抵抗型前列腺癌22RV1细胞增殖与凋亡的影响

Effects of Resveratrol on proliferation and apoptosis of castration-resistant prostate cancer 22RV1 cells

目的:探讨白藜芦醇(resveratrol,Res)对去势抵抗型前列腺癌(castration-resistant prostate cancer,CRPC)22RV1细胞增殖与凋亡的影响并探讨其可能机制。方法:用不同浓度Res作用于22RV1细胞24h后,在光学显微镜下观察细胞形态学变化。CCK-8法检测Res对细胞增殖的影响。应用Annexin-V/PI双标记法染色后,用流式细胞仪检测细胞凋亡情况。用活性氧特异性试剂盒检测活性氧含量。Western blot检测Bcl-2和Bax蛋白表达情况。结果:与对照组比较,Res可使22RV1细胞形态出现皱缩,变圆,细胞间隙增大,贴壁细胞减少,凋亡细胞增多。CCK-8结果示Res可抑制22RV1细胞增殖作用(P<0.05)。流式检测结果示Res可诱导22RV1细胞凋亡(P<0.01)。活性氧检测结果示Res可增加2 2 RV1细胞内活性氧含量(P<0.01),并呈剂量相关性。Western blot检测发现随Res浓度的增加,Bcl-2蛋白表达下降,Bax蛋白表达增加。结论:Res能够抑制22RV1细胞的增殖并诱导其凋亡。Res诱导22RV1细胞凋亡的机制可能与Res增加22RV1细胞内活性氧水平,激活促凋亡因子Bax,并抑制抗凋亡因子Bcl-2的活性,继而引发后续凋亡反应。

Objective：To observe the effect of resveratrol on proliferation and apoptosis of prostate cancer 22RV1 cells and explore the possible mechanisms.Methods：Ordinary optical inverted microscope was used to observe the morphological changes and cell proliferation was measured with a cell counting Kit-8.Apoptosis assays were performed by using the FITC Annexin V apoptosis detection Kit I by flow cytometry and Western blotting was used to analyze the expression of Bax and Bcl-2 protein in Resveratrol-treated 22RV1 cells.Accumulation of ROS was quantified by flow cytometry with a reactive oxygen species assay Kit.Results：Compared with the control group,the proliferation of 22RV1 was significantly inhibited by Res in a does and time-dependent manner（ P〈0.05）,and the apoptosis percentage in every experiment groups were higher（ P〈0.01）,at the same time,the total ROS level was increased,and the expression of Bcl-2 was downregulated,as the Bax was upregulated with the increase of the Res concentration.Conclusion：Res could inhibit the proliferation cell and induced apoptosis of 22RV1,the mechanism is related with modulating ROS level and the expression of Bcl-2 / Bax.