Livin蛋白在支气管肺癌中的表达及与Bcl-2的关系

Expression and correlation of Livin and Bcl-2 proteins in primary lung cancer

目的:探讨Livin蛋白在原发性支气管肺癌组织的表达及其与Bcl-2蛋白之间的关系。方法:采用免疫组化SP法检测Livin与Bcl-2在支气管肺癌组织和癌旁5cm以上正常肺组织中蛋白的表达情况。结果:肺癌组的Livin蛋白和Bcl-2蛋白阳性表达率均高于对照组(均P<0.05)。男性肺癌患者Livin蛋白的表达阳性率为62.96%,女性为40.00%,两者相比差异有统计学意义(P<0.05)。有淋巴结转移的肺组织中Livin蛋白和Bcl-2蛋白的阳性表达率较高(78.95%vs 36.36%,68.42%vs 27.27%,均P<0.05)。肺鳞癌组织Bcl-2基因蛋白的阳性表达率为58.82%,显著高于肺腺癌组织中Bcl-2基因蛋白表达(20.45%),差异有统计学意义(P<0.05)。吸烟指数≥400的肺癌患者Livin蛋白及Bcl-2蛋白表达阳性率均高于吸烟指数<400的患者,差异有统计学意义(P<0.05)。支气管肺癌的组织分化程度、临床分期对Livin蛋白和Bcl-2蛋白的阳性表达率的影响均无统计学差异。Livin蛋白与Bcl-2蛋白的表达呈正相关。结论:Livin蛋白与Bcl-2蛋白在支气管肺癌的异常表达,有望成为肿瘤诊断及基因治疗的新靶点。Livin蛋白与凋亡相关蛋白Bcl-2的异常表达在支气管肺癌癌变中有协调作用。

Objective：To study the expression of Livin and it＇s relationship with expression of Bcl-2 in primary lung cancer.Methods：The protein expression of Livin and Bcl-2 gene in paraffin specimens of lung cancer and specimens of normal lung tissues were detected by SP immunohistochemistry.Results：Livin and Bcl-2 protein expression showed high level in lung cancer specimens and were negative staining in the normal lung tissues.The positive rate of Livin protein expression in male lung cancer specimens（ 62.96%） was higher than that in female lung tissues（ 40.00%,P〈0.05）.The positive rates of Livin and Bcl-2 protein expression in specimens with lymph node metastasis（ 78.95% and 68.42%） were higher than in tissues without lymph node metastasis（ 36.36% and 27.27%,P〈0.05）.The protein expression of Bcl-2 gene in squamous cell carcinoma lung tissues（ 58.82%） was higher than that in adenocarcinoma lung tissues（ 20.45%）.The positive rates of expressions of Livin and Bcl-2 protein were higher in patients smoking index ≥400 than patients with 400,the differences were significant（ P〈0.05）.Livin protein expression in lung cancer had no obvious relationship with the grade and clinical stage.Livin protein expression was positively related to Bcl-2 protein expression in primary lung cancer.Conclusion：Aberrant expression may be a new target for diagnosis and gene treatment of primary lung cancer.The aberrant expression of Livin and apoptosis related gene Bcl-2 may play synergetic roles in process of carcinogenesis of lung cancer.