高效毛细管电泳法测定两种不同颜色拳参饮片中没食子酸含量

Determination of Gallic Acid in Quanshen Pieces of Two Different Colors by HPCE

目的建立毛细管电泳测定拳参中没食子酸的方法。检测紫红色和棕红色两种商品拳参饮片中没食子酸的含量是否有差异。方法采用高效毛细管电泳法,缓冲液为pH 9.0的20 mmol·L^(-1)硼砂缓冲溶液;运行电压为20 kV;检测波长为290 nm;进样压力5 kPa,进样时间10 s;运行温度为室温。结果整个分析过程在6 min内完成。湖北、山东、河北3个产地7个批次的拳参饮片中,紫红色拳参饮片没食子酸的含量:湖北2.70%,山东12.08%、7.79%,河北2.01%、5.78%、6.97%、4.22%;棕红色拳参饮片中没食子酸的含量:湖北1.35%,山东1.81%、2.94%,河北1.87%、3.42%、0.44%、1.52%。结论该方法简单,经济,快速,可以为拳参质量控制提供依据。同时,紫红色拳参饮片中没食子酸的含量高于棕红色饮片。

OBJECTIVE To establish a high-performance capillary electrophoresis （CE） method for determinating gallic acid in commercial Quanshen pieces of two different colors, amaranth and brownish red. METHODS CE-UV method was used in the follow- ing condition： BGE 20 mmol ~ L-l sodium borate solution, vohage 20 kV, detection wavelength 290 nm, sample injection 10 s at 5 kPa, capillary temperature room temperature. RESULTS A quantitative method was successfully developed to determine gallic acid in Quanshen pieces within 6 min. The contents of gallic acid in 7 batches of amaranth colored Quanshen pieces were 2.70% （ Hubei province）, 12. 08% and 7. 79% （ Shandong province）, 2. 01%, 5.78%, 6. 97%, and 4. 22% （ Hebei province）, respectively. The contents of gallic acid in brownish red colored Quanshen pieces were 1.35% （ Hubei province） , 1.81% and 3.42% （ Shandong prov- ince）, 1.87%, 3.42% , 0. 44%, and 1.52% （Hebei province）, respectively. CONCLUSION The method is rapid, economical and simple and can provide a powerful quality control measure for Quanshen pieces. The contents of gallic acid in the amaranth colored Quanshen pieces are higher than those in the brownish red colored pieces.