姜黄素协同顺铂对卵巢癌耐药细胞株COC1/DDP抑制作用的实验研究

Inhibitory effects of curcumin coordinated DDP on cisplatin-resistance human ovarian cancer line COC1/DDP

目的观察姜黄素协同顺铂对卵巢癌耐药细胞株COC1/DDP细胞的抑制作用的影响。方法采用MTT法检测实验组Ⅰ顺铂（1,1.5,2.5、5μg/ml）作用以及实验组Ⅱ顺铂（1,1.5,2.5、5μg/ml）个浓度与姜黄素（20μmol/L）联合作用于COC1/DDP细胞各48h并观察两实验组作用结果。用透射电镜观察姜黄素（25μmol/L）培养24h COC1/DDP细胞的超微结构。结果顺铂浓度在（1-5）μg/ml时加入姜黄素20μmol/L后,耐药细胞生存率曲线下降明显（P〈0.05）,尤以顺铂2.5μg/ml和姜黄素20μmol/L作用时耐药细胞抑制率提高最明显。单用DDP组细胞抑制为31.673±0.125（%）,DDP与姜黄素联用组细胞抑制为58.965±0.109（%）,两药联用与单独用DDP相比抑制细胞增殖作用显著增强（P〈0.05）。透射电镜观察姜黄素组作用24h后COC1/DDP细胞超微结构,可见细胞表面微绒毛消失,细胞核固缩,染色体凝集成新月状,形成凋亡小体。结论姜黄素可增强COC1/DDP对顺铂的敏感性,这可能与姜黄素和顺铂协同诱导并促进细胞凋亡发生有关。

Objective To investigate the effect and its mechanism of curcumin cooperated with DDP on the apoptosis sensitivity of the COC1/DDP. Methods With MTT（Methyl Thiazolyl Tetrazolium） method to study on the work result of COC1/DDP cells after 48 hours,which been done with Group Ⅰ DDP（1,1.5,2.5,5μg/ml） and with Group Ⅱ DDP（1,1.5,2.5、5μg/ml） that each concentration DDP combined with curcumin（20μmol/L）;Use the transmission electron microscope to observe the the ultramicro-fracture of COC1 DDP cell that after 24 h later influenced by curcumin（20μmol /L）. Results 1-5μg/ml DDP combined with 20μmol/L curcumin could decrease surviving rates of COC1/DDP cells（P〈0.05）,especially 2.5μg/ml DDP combined with 20μmol/L curcumin;The depressant effection of COC1/DDP cells with DDP combined with curcumin were significantly higher than that of only DDP and curcumin（P〈0.05）. 24 hours after the treatment of curcumin（20μmol/L） with COC1/DDP,We observed by transmission electron microscope that microvillus disappeared on the surface of COC1/DDP cells,cell-nucleus was condensed,and the chromosomal was been agglutinated crescent,apoptosis body had been formed. Conclusion curcumin can enhance the sensitivity of DDP to COC1/DDP,mechanism may be associated with the induction and promotion to the apoptosis of COC1/DDP by curcumin coordinated DDP.