线性扫描极谱法检测环境水样中四环素

Determination of Tetracycline in Environmental Water Samples by Linear Sweep Polarography

基于四环素在pH=5.5的柠檬酸-柠檬酸钠缓冲溶液中可产生一灵敏的还原峰,建立了一种测定四环素的极谱新方法。在优化条件下,于起始电位-0.9 V（vs.SCE）,峰电位-1.4V（vs.SCE）,四环素浓度分别在0.1~30、30~90μg/mL范围内与峰电流呈现良好的线性关系,检出限为0.0024μg/mL。该方法直接用于环境水样中四环素的检测,回收率为97.8%~102.9%。用线性扫描极谱法研究了检测体系的电化学行为,证明了极谱波为不可逆还原吸附波,并讨论了电极反应机理。

A method for the determination of tetracycline by polargraphy analysis has been developed. It＇s based on the reduction peak current of tetracycline in the citric acid buffer solution（pH 5. 5）. The starting potential is -0.9 V（vs. SCE）, and the peak potential is -1.4 V（vs. SCE）. The peak current is linearly proportional to the concentration of tetracycline over the range of 0.1-30 μg/mL and 30-90 μg/mL, and the detection limit is 0. 0024μg/mL. The recoveries for spiking sample analysis are from 97.78% to 102.9%. The method is simple,rapid and accurate and the result is satisfactory when it is used to analyze tetracycline in environmental water sample. The electrochemical behavior of tetracycline was studied, and the experimental results show that the reaction of tetracyline at the electrode had the characteristic of irreversible reductive adsorptive wave, and the mechanism of the electrode reaction is preliminarily discussed.