结直肠癌、癌前病变中KLF6 mRNA与蛋白表达的研究

KLF6 mRNA and protein expression levels in colorectal cancer and precancerous lesions

目的：检测抑癌基因KLF6（ kruppel-like factor 6）在结直肠癌、癌前病变（均结直肠高级别上皮内瘤变）及癌旁正常黏膜组织细胞中的表达水平，探讨KLF6表达水平在各类型组织中的相互关系及在结直肠癌病程中的作用。方法：采集弋矶山医院2014年1月～2015年1月间结直肠手术切除后标本共88例，其中癌组织46例、癌前病变（结直肠高级别上皮内瘤变）组织21例及正常肠管黏膜组织21例。以SP法进行免疫组化和Q-PCR方法检测结直肠癌（结直肠癌组）、结直肠癌癌前病变（癌前病变实验组）及癌旁组织（对照组）中KLF6表达水平；并探讨KLF6的表达与临床病理参数之间的关系。结果：①KLF6在结直肠癌组织、结直肠癌癌前病变组织中的表达水平低于癌旁正常组织（ P＜0．05），差异有统计学意义；且KLF6表达与浸润情况、分化程度、淋巴结转移、TNM分期有一定相关性（ P＜0．05）；KLF6表达与患者的年龄、性别、肿瘤组织学类型、癌灶大小无明显相关性（ P＞0．05）。②Q-PCR实验结果正常组织中KLF6基因相对表达量高于结直肠癌癌前组织和结直肠癌组织，差异有统计学意义（ P＜0．05）。结论：KLF6基因在结直肠癌及癌前病变中起到了一定的作用，并与肿瘤浸润情况、分化程度、淋巴结转移、TNM分期有一定相关性。

Objective： To investigate the association and role of Kruppel-like factor 6（ KLF6） gene and protein expression with precancerous lesions and in the development of colorectal cancers via detecting the levels of the gene and protein in the malignant and adjancent tissues .Methods：The samples were collected in 88 patients with colorectal cancer treated in our hospital from 2014 through 2015.Of the 88 samples,46 were from cancer tissues,21 from pre-cancerous lesions and another 21 from adjacent normal colorectal mucosa .KLF6 mRNA and protein expression levels were detected with immunohistochem-istry and quantitative polymerase chain reaction （Q-PCR） in the tissues of colorectal cancer（colorectal cancer group）,precancerous lesions（precancerous lesions group） and in tumor-adjacent tissues（control group）,and the association of KLF6 expression with clinical pathological parameters were also deter-mined.Results：① KLF6 expression was significantly lower in either of the tissues from colorectal cancer or precancerous lesions than that of tumor-adjacent normal tissues（ P〈0.05） ,and was somewhat correlated with the extent of tumor infiltration ,differentiation,lymph node metastasis and TNM staging （ P〈0.05）,yet was not significantly with the patient′s age,gender,histological type and tumor size（P〉0.05）;②Q-PCR indicated that KLF6 gene expression was relatively higher than the level in either tissues from precancerous lesions or from colorectal cancers .The difference was significant （ P〈0.05 ） . Conclusion：KLF6 gene can be important role in colorectal cancer and precancerous lesions,and is associated to a certain extent with tumor infiltration ,dif-ferentiation,lymph node metastasis and TNM staging.