结合珠蛋白基因多态性与未足月胎膜早破的临床研究

Clinical study of haptoglobin polymorphism with preterm premature rupture of membranes

目的:探讨结合珠蛋白(haptoglobin,Hp)基因多态性与未足月胎膜早破的关系,为未足月胎膜早破的遗传病因学提供理论依据。方法:应用序列特异性引物聚合酶链反应(PCR-SSP)进行基因分型检测,分析Hp基因型Hp1-1型、Hp2-1型及Hp2-2型在正常足月分娩孕妇组(对照组)和未足月胎膜早破孕妇组(研究组)的分布。结果:等位基因Hp1和Hp2在正常孕妇和未足月胎膜早破患者的分布频率分别为39.06%、60.94%和28.0%、72.0%,两组等位基因频率和基因型频率分布差异无统计学意义(P>0.05)。结论:在中国汉族人群中,结合珠蛋白基因多态性与未足月胎膜早破发生无相关性,其基因型分布不是中国汉族孕妇未足月胎膜早破发病原因。

Objective：To investigate the association of allelic polymorphism of haptoglobin（Hp）with preterm premature rupture of membrane（PPROM） for genetic evidence leading to PPROM.Methods：PCR techniques with sequence specific primers（SSP） was used to detect genotype Hp1-1,Hp2-1and Hp2-2 in human Hp gene and their distribution in normal pregnancy and PPROM.Results：The frequencies of Hp1 and HP2 alleles were 39.06%and 60.94%for the normal pregnancies,and 28% and 72% for the test group,respectively.There was no significant difference in the genotype distribution （P〉0.05）and allelic frequencies （P〉0.05） between groups.Conclusion：The findings showed no association of haptoglobin polymorphism with PPROM, suggesting that this gene distribution may not be the risk factor of pregnant women in Han population complicated with PPROM .