研究HB-EGF、C-erbB-2检测对于卵巢良恶性肿瘤鉴别诊断以及判断病情复杂程度的意义

目的：研究HB-EGF、C-erbB-2检测对于卵巢良恶性肿瘤鉴别诊断以及判断病情复杂程度的意义.方法：收集我院 2012 年 6 月至2013年 5 因卵巢肿瘤行手术治疗的患者80例,其中良性卵巢瘤各20例,上皮性卵巢癌40例,其中在上皮性卵巢癌中,Ⅰ期13例,Ⅱ期14例,Ⅲ-Ⅳ期13例,另取30例正常子宫内膜志愿者的卵巢组织,采用组织流式细胞术检测HB-EGF、C-erbB-2在卵巢组织中的表达情况;采用免疫组织化学法检测40例子宫内膜癌、30例正常子宫内膜中c-erbB-2的表达.结果：HB-EGF、C-erbB-2在卵巢癌组织中阳性细胞率显著高于良性卵巢瘤,差异具有统计学意义（P〈0.05）;CerbB-2 在正常子宫内膜为低表达,在子宫内膜癌中的含量比正常子宫表达增高,差异有统计学意义（P〈0.05）;子宫内膜癌的病理分期不同,C-erbB-2 表达也不同,其中子宫内膜癌中CerbB-2 的表达中Ⅰ期、Ⅱ期明显低于Ⅲ、Ⅳ期,差异有统计学意义（P〈0.05）.结论：HB-EGF、C-erbB-2检测可作为卵巢良恶性肿瘤鉴别诊断的辅助指标,C-erbB-2 蛋白高表达与子宫内膜癌的发生、发展有关,CerbB-2 的检测有助于临床上评估子宫内膜癌的生物学行为和预后.

Objective： To study HB-EGF, C - erbB - 2 test in differential diagnosis of ovarian benign and malignant tumor and determine the meaning of a complex condition. Methods： Collected from June 2012 to May 2013 for surgical treatment of 80 cases of patients with ovarian tumors, benign ovarian tumor and 20 cases of epithelial ovarian cancer 40 cases, among them in epithelial ovarian cancer, 13 cases of Ⅰ period, Ⅱ period in 14 cases, Ⅲ ~ Ⅳ period 13 cases, the other 30 cases of normal endometrium volunteers ovarian tissue, using flow cytometry detection organization HB EGF, C - erbB - 2 expression in ovarian tissue; The immune histochemical approach to detect 40 examples carcinoma, 30 cases of normal endometrium intrauterine membrane in c - erbB - 2 expression. Results： HB-EGF, C - erbB - 2 in the rate of positive cells in ovarian cancer tissues was significantly higher than that of benign ovarian tumor, statistically significant difference （P 〈 0.05）; CerbB - 2 for lower expression in normal endometrium, endometrial carcinoma in content is higher than the normal uterus express, the difference was statistically significant （P 〈 0.05）; Endometrial cancer of different pathological staging, C - erbB - 2 expression is different also, including CerbB - 2 expression in endometrial carcinoma of stage Ⅰ, Ⅱ significantly below Ⅲ, Ⅳ period, the difference was statistically significant （P 〈 0.05）. Conclusion： HB-EGF, C - erbB - 2 testing can be used as auxiliary indexes in the differential diagnosis of benign and malignant tumor, ovary C - high erbB - 2 protein expression is associated with the occurrence and development of endometrial carcinoma, CerbB - 2 testing helps to clinically evaluate the biological behavior and prognosis of endometrial carcinoma.