内毒素诱导HepG2凋亡的研究

目的：探讨内毒素对HepG2细胞的直接作用,为肝细胞损害的防治研究提供新的模型.方法：利用HepG2细胞作为体外肝细胞模型,HepG2细胞经不同浓度LPS（0.1、10、20μg/ml、作用24、48小时, 应用流式细胞仪测定细胞凋亡率,分光光度计法测 Caspase-3活性;ELISA 法检测细胞培养上清 TNF-α;结果：不同浓度内毒素刺激后 TNF-α、Caspase-3明显升高.结论：LPS直接引起 HepG2 细胞凋亡,并呈浓度依赖性.

Objective ：In order to investigate the effect of LPS on human HepG2 cells , HepG2 cells was treated with different LPS （0.1、10、20μg/ml） for 24、48hours , The hepatocyte apoptosis was detected by theTUNEL method, Spectrophotometer method detected Caspase-3 activity. Cell culture supernatant TNF-α was detected by ELISA assay. Results The change of TNF-α and Caspase-3 in the dose-effect relationship compared with control group had significantly difference under stimulation of endotoxin （p〈0.05）. Conclusion ： LPS could induce apoptosis of HepG2 cells .