高效凝胶色谱法测定贻贝多糖含量及分子量

Determination of the content and the molecular weight of Mussel Polysaccharide by GPC

目的建立使用凝胶色谱测定贻贝多糖含量和分子量的方法。方法使用岛津高效液相色谱,TSK-gel GMPWXL(7.8mm×300 mm)凝胶色谱柱,以0.05 mol/m L硝酸钠溶液(含0.05%叠氮钠)做为流动相,柱温35℃,流速为0.6 m L/min,RID-20AT示差折光检测器,检测池温40℃。结果对所建立的分析方法进行精密度、稳定性、重复性以及回收率等方法学研究。经测定厚壳贻贝多糖平均含量88.6%,平均重均分子量为1 261 411;紫贻贝多糖平均含量87.4%,平均重均分子量为1 244 062。结论本文所用方法各项指标均符合分析要求,且具有快速可靠、灵敏度高和重现性好等特点,可用于贻贝多糖的质量控制。

Objective To develop a gel permeation chromatography method for determination of content and molecular weight（ Mw） of Mussel Polysaccharide. Methods Using GPC method,the sample was separated with TSK-gel GMPWXL（ 7. 8 mm × 300 mm） chromatography column which was set at 35 ℃. The mobile phase was 0. 05 mol/m L Na NO3（ including 0. 05% Na2N3） and the flow rate was 0. 6 m L/min. The detector was RID-20 AT.Results The average molecular weight of the polysaccharide of Mytilus coruscus was 1 261 411 and the average content was 88. 6% by using of the calibration curves of dextrans. The average molecular weight of the polysaccharide of Mytilus edulis was 1 244 062 and the average content was 87. 4%.Conclusion The method established in this paper is simple and rapid,accurate and reproducible,which can be used for the quality control of Mussel Polysaccharide.