miRNA-9对骨肉瘤MG-63细胞增殖、凋亡的作用及其机制

The effects of microRNA-9 on proliferation and apoptosis of osteosarcoma MG-63 cells and correlated mechanisms

目的:通过提高骨肉瘤MG-63细胞miRNA-9的表达,探讨miRNA-9对MG-63细胞增殖及凋亡的影响及可能的作用机制。方法:MG-63细胞随机分为实验组、阴性对照组和空白组。实验组转染miRNA-9(Oligo),阴性对照组转染阴性对照核苷酸序列(microRNA negative control sequence),空白组不做转染。qRT-PCR检测细胞miRNA-9、CXCR4的表达。CCK-8法检测3组细胞的增殖;流式细胞术比较3组细胞的凋亡水平。结果:与阴性对照组和空白组相比,转染组细胞中miRNA-9表达升高;miRNA-9高表达的骨肉瘤细胞增殖速率降低、细胞凋亡率增加、CXCR4 mRNA转录水平下调,差异有统计学意义。结论:CXCR4基因参与miRNA-9抑制骨肉瘤MG-63细胞增殖过程,同时促进细胞凋亡。

Objective： To study the function of miRNA-9 on osteosarcoma MG-63 cells proliferation and apoptosis and correlated mechanisms by improving the expression of miRNA-9. Methods： Osteosarcoma MG-63 cells were randomly divided into three groups： Experimental group（ transfected with miRNA-9 Oligo）,control group（ transfected with miRNA negative contrat） and blank group（ transfected nothing）. Detected the expression of miRNA-9,CXCR4 by real time PCR. Detected the CCK value（ OD） at 24 h,48h,72 h and 96 h,then observed the cell growth curve. The apoptosis was measured by flow cytometry. Results： Compared with negative control group and blank group,the miRNA transfection group was higher expressed on miRNA-9,osteosarcoma cells showed lower cell proliferation efficiency and higher cell apoptosis rotio on miRNA-9 higher expression groups. Conclusion： CXCR4 gene involved in the miRNA-9 inhition of osteosarcoma MG-63 cell proliferation,and promote cell apoptosis.