摘要
目的:观察在不同卵巢组织中miRNA-22的表达,并研究上调miRNA-22表达对卵巢癌细胞株SKOV-3增殖、迁移与侵袭的影响。方法:实时荧光定量PCR(q PCR)检测不同卵巢组织样本miRNA-22的表达;随机将SKOV-3细胞分为正常对照组(control组)、空白载体组(miRNA-22-NC组)和转染miRNA-22-mimic组(miRNA-22-m组),q PCR检测各组细胞miRNA-22的表达,CCK-8法检测细胞存活率,划痕实验与Transwell小室法分别检测细胞的迁移与侵袭能力,Western blot法检测VEGF和P53蛋白表达。结果:卵巢癌组织中miRNA-22的水平显著低于正常卵巢组织;与control组相比,miRNA-22-m组细胞miRNA-22的表达显著增加,细胞存活率显著降低,细胞迁移数和侵袭数下降,VEGF和P53的蛋白表达水平显著下降。结论:miRNA-22的低表达可能与卵巢癌的发生发展有关。过表达miRNA-22能够抑制卵巢癌细胞株的增殖、迁移和侵袭,其机制可能与下调VEGF和P53的蛋白表达有关。
AIM:To examine the expression of miRNA-22 in the ovarian tissues and the effect of miRNA-22over-expression on the proliferation,migration and invasion in SKOV-3 cells.METHODS:The expression levels of miRNA-22 in different ovarian tissues and SKOV-3 cells were determined by q PCR.miRNA-22 was over-expressed by transfection of miRNA-22 mimic.The cell viability was examined by CCK-8 assay.The cell migration was measured by wound healing test.The cell invasion was analyzed by Transwell assay.The protein expression levels of VEGF and P53 were determined by Western blot.RESULTS:Compared with the normal ovarian tissue,the expression level of miRNA-22 was remarkably decreased in the ovarian tumor tissues.After transfection with miRNA-22 mimic,the expression level of miRNA-22 in the SKOV-3 cells was significantly increased,while the cell viability,migration and invasion were obviously decreased.Moreover,the protein expression of VEGF and P53 was dramatically inhibited after over-expression of miRNA-22.CONCLUSION:The decreased miRNA-22 expression may be correlated with the development of ovarian cancer.Over-expression of miRNA-22 decreases the cell viability,migration and invasion by reducing the protein expression of VEGF and P53.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第12期2251-2255,共5页
Chinese Journal of Pathophysiology