低聚果糖和低聚异麦芽糖促进斑马鱼肠道蠕动作用的研究

The effect of fructo oligosaccharides and isomaltooligosaccharide on the intestinal peristalsis of zebrafish

探讨基于斑马鱼模型的低聚果糖及低聚异麦芽糖对肠道蠕动的促进作用.选用发育正常的5d的斑马鱼幼鱼,各实验组分别加入不同浓度的低聚果糖和低聚异麦芽糖溶液,培养24h后,在显微镜下观察其对斑马鱼肠道蠕动次数的影响.实验结果表明,125-500mg/mL的低聚果糖和低聚异麦芽糖均对斑马鱼肠道蠕动有促进作用,蠕动次数与浓度成正相关性.相同浓度条件下,低聚果糖较低聚异麦芽糖促进斑马鱼肠道蠕动的作用更明显.采用斑马鱼动物模型可实现促进肠蠕动药物的早期、快速筛选.

The effect of fructo oligosaccharides and isomaltooligosaccharide in different concentration to promote the intestinal peristalsis is discussed by using zebrafish. We selected the 5 d normal growth zebrafish larvae, and then added to a series of fructo oligosaccharides or isomaltooligosaccharide with a range of concentration as experiment group. The number of intestinal peristalsis was counted in all groups including control group and oligosaccharide and oligoisomaltose treated groups with different dose under the microscope after 24 h. The result showed that fructo oligosaccharides and isomaltooligosaccharide can promote zebrafish larval intestinal peristalsis in the concentration of 125-500 mg/mL in dose- dependent manner. The effect of oligosaccharide was more obvious than oligoisomaltose under same dose. Using zebrafish can achieve early and rapid screening for drugs promoting intestinal peristalsis.