高通量测序与传统纯培养方法在牡蛎微生物群落分析中的应用对比

Microbial Flora Analysis of Oyster: A Comparison between Traditional Plate Culture Method and High Throughput Sequencing

为揭示牡蛎体附着的微生物群落结构特征,采用Illumina Hi Seq高通量测序技术对牡蛎的细菌种类进行了分析,并与传统纯培养方法的分析结果进行对比。结果表明,牡蛎体附着的细菌以变形菌门(Proteobacteria,77.5%)、γ-变形菌纲(Gammaproteobacteria,72.3%)为主;在目的分类水平上,弧菌目(Vibrionales,49.3%)、交替单胞菌目(Alteromonadales,12.2%)占优势;在科的分类水平上,主要是弧菌科(Vibrionaceae,36.8%)、希瓦氏菌科(Shewanellaceae,10.3%)和交替假单胞菌科(Pseudoalteromonadaceae,7.2%);在属的分类水平上,弧菌属(Vibrio,28.3%)、希瓦氏菌属(Shewanella,10.3%)、交替假单胞菌属(Pseudoalteromonas,7.2%)比例相对较高。高通量测序分析结果中,丰度前3位的菌属与纯培养鉴定的结果一致,但比例有所差异。

Illumina Hi Seq high throughput sequencing and the traditional plate culture method were comparatively applied to investigate the structure of bacterial community attached to oyster.Results showed that the dominant bacteria attached to oyster belonged to the Gammaproteobacteria（72.3%） class in the Proteobacteria（77.5%） phylum.At the order level,Vibrionales（49.3%） and Alteromonadales（12.2%） were dominant.At the family level,Vibrionaceae（36.8%）,Shewanellaceae（10.3%） and Pseudoalteromonadaceae（7.2%） were dominant.At the genus level,the relative proportions of Vibrio（28.3%）,Shewanella（10.3%） and Pseudoalteromonas（7.2%） were high.The results of high throughput sequencing for the top three most dominant genera were consistent with those obtained with the traditional culture method although the proportions of these bacteria were somehow different.