高效液相色谱检测番茄与土壤中的吩胺霉素残留

Determination of New Pesticide Phenazine-1-carboxamide Residue in Tomato and Soil by HPLC with DAD

吩胺霉素是我国具有自主知识产权的新型抗生素类杀菌剂．选取番茄及三类理化性质不同的土壤为研究对象，建立了吩胺霉素在上述基质中的液相色谱检测方法．土壤中吩胺霉素选择混合液（V氨水：V乙醇：V二氯甲烷=1：2：3）振荡提取，番茄中吩胺霉素采用乙腈振荡提取，碱性氧化铝柱净化．土壤与番茄中的平均回收率分别为80．2％～95．7％和80．1％～85．6％，RSD（相对标准偏差）分别为2．14％～4．17％和4．12％～5．17％．吩胺霉素的最小检出量为0．2ng，在基质中最低检测限为0．01mg／kg．本方法简单可靠，基质效应弱，符合农药残留分析要求，可用于土壤和番茄中吩胺霉素的残留检测．

Phenazine-l-carboxamide is a new pesticide. This paper evaluates the residue of phenazine-1- carboxamide in tomato and three kinds of soils. New methods for determining phenazine-l-carboxamide residue in these matrices were developed by high performance liquid chromatography detection. Phenazine- 1-carboxamides from soil with ammonia water ＋ ethanol ＋ dichloromethane = 1 ＋2 ＋3 （v/v） and from tomato with acetonitrile were exacted, followed by chromatography columns （basic aluminum） cleanup. The mean recoveries of phenazine-l-carboxamide in soil and tomato were 80.2% to 95.7% and 80.1% to 85.6~~ respectively, while the relative standard deviations were 2.14% to 4.17% and 4.12% to 5.17%, respectively. The minimum detectable quantity of phenazine-l-carboxamide was 0.2 ng, and the limit ofdetection was 0. 01 mg/kg. In a whole, this method is simple and reliable, and has weak matrix effects, which satisfies the requirement of pesticide residue analysis. It is applicable to determine the phenazine-1- carboxamide residues in soil and tomato.