苏拉明对脓毒症大鼠炎症反应的调控作用研究

Regulation of suramin on inflammatory response of septic rat

目的探讨苏拉明对脓毒症大鼠模型炎症反应的调控作用,为临床治疗提供参考依据。方法将36只SD大鼠分为苏拉明组和对照组,各18只,建立脓毒症大鼠动物模型,ELISA法测定两组大鼠肺组织和血清白细胞介素-6(IL-6)和肿瘤坏死因子-a(TNF-a)水平。结果建模后两组大鼠肺组织和血清IL-6、TNF-a水平均高于建模前(P<0.05),建模后1天,苏拉明组肺组织和血清IL-6、TNF-a水平均低于对照组(P<0.05),建模后3天,两组肺组织和血清IL-6、TNF-a比较差异无统计学意义;单核细胞THP-1细胞在LPS作用1h时,苏拉明组plB相对表达量低于对照组(P<0.05),苏拉明组P65相对表达量高于对照组(P<0.05)。结论苏拉明可以减轻脓毒症大鼠的炎症反应,对脓毒症大鼠的肺损伤有保护作用,其减轻炎症反应的机制可能和抑制单核细胞NF-κB活性有关。

OBJECTIVE To explore the regulation effect of suramin on inflammatory response of septic rat,so as to provide references for clinical treatment.METHODS The 36 SD rats were divided into suramin group and control group,with 18 cases in each group,and septic rat model was established.The lung tissue and serum levels of interleukin-6（IL-6）and tumor necrosis factor-a（TNF-a）in the two group of rats were detected by ELISA.RESULTS After modeling,the lung tissue and serum levels of IL-6and TNF-a in the two groups of rats were higher than those of before modeling（P〈0.05）.One day after modeling,the lung tissue and serum levels of IL-6and TNF-a of suramin group were lower than those of control group（P〈0.05）,and 3days after modeling,the difference of lung tissue and serum levels of IL-6and TNF-a in the two groups was not significant.In monocytic THP-1cells after one hour of LPS functioning,the relative expression of plκB of suramin group was lower than that of control group（P〈0.05）,the relative expression of P65 of suramin group was higher than that of control group（P〈0.05）.CONCLUSIONSuramin may reduce inflammation response in septic rats,and has protective effect on acute lung injury in septic rats,its mechanism of reducing inflammation response may relate with inhibition of monocyte NF-κB activity.