牙周膜干细胞膜片构建的生物性牙根生物学活性研究

Biological activity of biological roots constructed by periodontal ligament stem cell sheet

目的将经过鉴定的人牙周膜干细胞(hPDLSC)膜片分别与支架材料预处理牙本质片(TDM)和羟基磷灰石/磷酸三钙(HA-TCP)进行体外共培养,评价不同支架对干细胞分化的影响。方法将单层牙周膜干细胞膜片与2种不同支架材料TDM和HA-TCP分别共培养1周,植入裸鼠皮下,通过扫描电镜和组织学动态观察复合体显微结构变化。结果细胞膜片与TDM边界处结合更为紧密;边界大量矿物沉积和纤维样组织生成;而在HA-TCP组只有单一的走形相对规则的纤维组织生成,未有明显矿化沉积生成。结论复合的hPDLSC/TDM组更适合具有生物学特点的干细胞发挥其特性,在合适的微环境下使hPDLSC向成牙周组织方向定向分化。

Objective To co-culture the identified human periodontal ligament stem cell membranes （hPDLSC） respectively with pretreated dentin matrix （TDM） and hydroxyapatite /tricalcium （HA-TCP） , and evaluate the effects of different scaffolds on stem cell differentiation. Methods Monolayer periodontal ligament stem cell membrane was incubated with two different scaffold materials for 1 week, and implanted into osteoporosis rats. Using scanning electron microscopy and histological section, the microsture changes were dynamically observed. Results A lot of mineral deposition and fibrous tissue were formed in the dentin matrix （TDM） group and there was some irregular fibrous tissue regeneration in the HA-TCP group without obvious mineral material generation. Conclusions hPDLSC/TDM composite is more suitable for the stem cells to fully develope their own biological characteristics. It can be more suitable for hPDLSC to differentiate into periodontium in suitable microenvironment.